Generation of iPSC lines from primary human amniotic fluid cells

By means of retroviral transduction using the four Yamanaka-factors OCT4, SOX2, KLF4 and c-MYC primary human amniotic fluid cells (AFCs) were reprogrammed into several iPSC lines. Pluripotency was confirmed both in vitro and in vivo. A comparative transcriptome analysis of the AF-derived iPSC line 4...

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Bibliographic Details
Main Authors: Katharina Drews, Peggy Matz, James Adjaye
Format: Article
Language:English
Published: Elsevier 2015-11-01
Series:Stem Cell Research
Online Access:http://www.sciencedirect.com/science/article/pii/S1873506115001567