Generation of iPSC lines from primary human amniotic fluid cells

Generation of iPSC lines from primary human amniotic fluid cells

By means of retroviral transduction using the four Yamanaka-factors OCT4, SOX2, KLF4 and c-MYC primary human amniotic fluid cells (AFCs) were reprogrammed into several iPSC lines. Pluripotency was confirmed both in vitro and in vivo. A comparative transcriptome analysis of the AF-derived iPSC line 4...

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Main Authors: Katharina Drews, Peggy Matz, James Adjaye
Format: Article
Language:English
Published: Elsevier 2015-11-01
Series:Stem Cell Research
Online Access:http://www.sciencedirect.com/science/article/pii/S1873506115001567
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spelling doaj-ea83013bd72e4c18ac53272cbbc95c852020-11-24T21:01:31ZengElsevierStem Cell Research1873-50611876-77532015-11-0115371271410.1016/j.scr.2015.11.003Generation of iPSC lines from primary human amniotic fluid cellsKatharina Drews0Peggy Matz1James Adjaye2Max Planck Institute for Molecular Genetics, 14195, Berlin, GermanyMax Planck Institute for Molecular Genetics, 14195, Berlin, GermanyMax Planck Institute for Molecular Genetics, 14195, Berlin, GermanyBy means of retroviral transduction using the four Yamanaka-factors OCT4, SOX2, KLF4 and c-MYC primary human amniotic fluid cells (AFCs) were reprogrammed into several iPSC lines. Pluripotency was confirmed both in vitro and in vivo. A comparative transcriptome analysis of the AF-derived iPSC line 41 and the human embryonic stem cell lines (H1 and H9) revealed a Pearson correlation of 0.953 and 0.941 respectively.http://www.sciencedirect.com/science/article/pii/S1873506115001567
collection DOAJ
language English
format Article
sources DOAJ
author Katharina Drews
Peggy Matz
James Adjaye
spellingShingle Katharina Drews
Peggy Matz
James Adjaye
Generation of iPSC lines from primary human amniotic fluid cells
Stem Cell Research
author_facet Katharina Drews
Peggy Matz
James Adjaye
author_sort Katharina Drews
title Generation of iPSC lines from primary human amniotic fluid cells
title_short Generation of iPSC lines from primary human amniotic fluid cells
title_full Generation of iPSC lines from primary human amniotic fluid cells
title_fullStr Generation of iPSC lines from primary human amniotic fluid cells
title_full_unstemmed Generation of iPSC lines from primary human amniotic fluid cells
title_sort generation of ipsc lines from primary human amniotic fluid cells
publisher Elsevier
series Stem Cell Research
issn 1873-5061
1876-7753
publishDate 2015-11-01
description By means of retroviral transduction using the four Yamanaka-factors OCT4, SOX2, KLF4 and c-MYC primary human amniotic fluid cells (AFCs) were reprogrammed into several iPSC lines. Pluripotency was confirmed both in vitro and in vivo. A comparative transcriptome analysis of the AF-derived iPSC line 41 and the human embryonic stem cell lines (H1 and H9) revealed a Pearson correlation of 0.953 and 0.941 respectively.
url http://www.sciencedirect.com/science/article/pii/S1873506115001567
work_keys_str_mv AT katharinadrews generationofipsclinesfromprimaryhumanamnioticfluidcells
AT peggymatz generationofipsclinesfromprimaryhumanamnioticfluidcells
AT jamesadjaye generationofipsclinesfromprimaryhumanamnioticfluidcells
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