CRISPR/Cas9-mediated gene knockout in human adipose stem/progenitor cells

CRISPR/Cas9-mediated gene knockout in human adipose stem/progenitor cells

The CRISPR/Cas9 system is a powerful tool to generate a specific loss-of-function phenotype by gene knockout (KO). However, this approach is challenging in primary human cells. In this technical report, we present a reliable protocol to achieve a functional KO in the genome of human adipose stem/pro...

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Bibliographic Details
Main Authors: Markus Mandl, Heike Ritthammer, Asim Ejaz, Sonja A. Wagner, Florian M. Hatzmann, Saphira Baumgarten, Hans P. Viertler, Marit E. Zwierzina, Monika Mattesich, Valerie Schiller, Tina Rauchenwald, Christian Ploner, Petra Waldegger, Gerhard Pierer, Werner Zwerschke
Format: Article
Language:English
Published: Taylor & Francis Group 2020-01-01
Series:Adipocyte
Subjects:
adipose stem cells
ageing
crispr/cas9
genome editing
loss-of-function
obesity
Online Access:http://dx.doi.org/10.1080/21623945.2020.1834230

Internet

http://dx.doi.org/10.1080/21623945.2020.1834230

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