Effects of Enzyme Loading and Immobilization Conditions on the Catalytic Features of Lipase From Pseudomonas fluorescens Immobilized on Octyl-Agarose Beads

The lipase from Pseudomonas fluorescens (PFL) has been immobilized on octyl-agarose beads under 16 different conditions (varying pH, ionic strength, buffer, adding some additives) at two different loadings, 1 and 60 mg of enzyme/g of support with the objective of check if this can alter the biocatal...

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Bibliographic Details
Main Authors: Sara Arana-Peña, Nathalia S. Rios, Diego Carballares, Carmen Mendez-Sanchez, Yuliya Lokha, Luciana R. B. Gonçalves, Roberto Fernandez-Lafuente
Format: Article
Published: Frontiers Media S.A. 2020-02-01
Series:Frontiers in Bioengineering and Biotechnology
Online Access:https://www.frontiersin.org/article/10.3389/fbioe.2020.00036/full