Escherichia coli σ70 promoters allow expression rate control at the cellular level in genome-integrated expression systems

Escherichia coli σ70 promoters allow expression rate control at the cellular level in genome-integrated expression systems

Abstract Background The genome-integrated T7 expression system offers significant advantages, in terms of productivity and product quality, even when expressing the gene of interest (GOI) from a single copy. Compared to plasmid-based expression systems, this system does not incur a plasmid-mediated...

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Bibliographic Details
Main Authors: Artur Schuller, Monika Cserjan-Puschmann, Christopher Tauer, Johanna Jarmer, Martin Wagenknecht, Daniela Reinisch, Reingard Grabherr, Gerald Striedner
Format: Article
Language:English
Published: BMC 2020-03-01
Series:Microbial Cell Factories
Subjects:
Recombinant protein expression
Escherichia coli
LacI autoregulation
Tunable expression
σ70 promoters
Genome-integrated expression systems
Online Access:http://link.springer.com/article/10.1186/s12934-020-01311-6

Internet

http://link.springer.com/article/10.1186/s12934-020-01311-6

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