A Comparative Study of Impedance versus Optical Label-Free Systems Relative to Labelled Assays in a Predominantly Gi Coupled GPCR (C5aR) Signalling

A Comparative Study of Impedance versus Optical Label-Free Systems Relative to Labelled Assays in a Predominantly Gi Coupled GPCR (C5aR) Signalling

Profiling ligand function on G-protein coupled receptors (GPCRs) typically involves using transfected cells over-expressing a target of interest, a labelled ligand, and intracellular secondary messenger reporters. In contrast, label-free assays are sensitive enough to allow detection in native cells...

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Bibliographic Details
Main Authors: Reena Halai, Daniel E. Croker, Jacky Y. Suen, David P. Fairlie, Matthew A. Cooper
Format: Article
Language:English
Published: MDPI AG 2012-07-01
Series:Biosensors
Subjects:
GPCR
impedance and optical biosensors
label-free
secondary messenger
signal transduction
Online Access:http://www.mdpi.com/2079-6374/2/3/273

Internet

http://www.mdpi.com/2079-6374/2/3/273

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