A Comparative Study of Impedance versus Optical Label-Free Systems Relative to Labelled Assays in a Predominantly Gi Coupled GPCR (C5aR) Signalling
Profiling ligand function on G-protein coupled receptors (GPCRs) typically involves using transfected cells over-expressing a target of interest, a labelled ligand, and intracellular secondary messenger reporters. In contrast, label-free assays are sensitive enough to allow detection in native cells...
Main Authors: | , , , , |
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Format: | Article |
Language: | English |
Published: |
MDPI AG
2012-07-01
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Series: | Biosensors |
Subjects: | |
Online Access: | http://www.mdpi.com/2079-6374/2/3/273 |