CRISPR/Cas9-mediated genome editing induces exon skipping by alternative splicing or exon deletion

CRISPR/Cas9-mediated genome editing induces exon skipping by alternative splicing or exon deletion

Abstract CRISPR is widely used to disrupt gene function by inducing small insertions and deletions. Here, we show that some single-guide RNAs (sgRNAs) can induce exon skipping or large genomic deletions that delete exons. For example, CRISPR-mediated editing of β-catenin exon 3, which encodes an aut...

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Bibliographic Details
Main Authors: Haiwei Mou, Jordan L. Smith, Lingtao Peng, Hao Yin, Jill Moore, Xiao-Ou Zhang, Chun-Qing Song, Ankur Sheel, Qiongqiong Wu, Deniz M. Ozata, Yingxiang Li, Daniel G. Anderson, Charles P. Emerson, Erik J. Sontheimer, Melissa J. Moore, Zhiping Weng, Wen Xue
Format: Article
Language:English
Published: BMC 2017-06-01
Series:Genome Biology
Online Access:http://link.springer.com/article/10.1186/s13059-017-1237-8

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http://link.springer.com/article/10.1186/s13059-017-1237-8

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