Decontamination of ambient RNA in single-cell RNA-seq with DecontX
Abstract Droplet-based microfluidic devices have become widely used to perform single-cell RNA sequencing (scRNA-seq). However, ambient RNA present in the cell suspension can be aberrantly counted along with a cell’s native mRNA and result in cross-contamination of transcripts between different cell...
Main Authors: | , , , , , , |
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Format: | Article |
Language: | English |
Published: |
BMC
2020-03-01
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Series: | Genome Biology |
Subjects: | |
Online Access: | http://link.springer.com/article/10.1186/s13059-020-1950-6 |