Decontamination of ambient RNA in single-cell RNA-seq with DecontX

Decontamination of ambient RNA in single-cell RNA-seq with DecontX

Abstract Droplet-based microfluidic devices have become widely used to perform single-cell RNA sequencing (scRNA-seq). However, ambient RNA present in the cell suspension can be aberrantly counted along with a cell’s native mRNA and result in cross-contamination of transcripts between different cell...

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Bibliographic Details
Main Authors: Shiyi Yang, Sean E. Corbett, Yusuke Koga, Zhe Wang, W Evan Johnson, Masanao Yajima, Joshua D. Campbell
Format: Article
Language:English
Published: BMC 2020-03-01
Series:Genome Biology
Subjects:
Bayesian mixture model
Decontamination
Single cell
scRNA-seq
Online Access:http://link.springer.com/article/10.1186/s13059-020-1950-6

Internet

http://link.springer.com/article/10.1186/s13059-020-1950-6

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