Rapid RNA analysis of individual Caenorhabditis elegans

Traditional RNA extraction methods rely on the use of hazardous chemicals such as phenol, chloroform, guanidinium thiocyanate to disrupt cells and inactivate RNAse simultaneously. RNA isolation from Caenorhabditis elegans presents another challenge due to its tough cuticle, therefore several repeate...

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Bibliographic Details
Main Authors: Kien Ly, Suzanne J. Reid, Russell G. Snell
Format: Article
Language:English
Published: Elsevier 2015-01-01
Series:MethodsX
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S2215016115000059