Rapid RNA analysis of individual Caenorhabditis elegans
Traditional RNA extraction methods rely on the use of hazardous chemicals such as phenol, chloroform, guanidinium thiocyanate to disrupt cells and inactivate RNAse simultaneously. RNA isolation from Caenorhabditis elegans presents another challenge due to its tough cuticle, therefore several repeate...
Main Authors: | , , |
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Format: | Article |
Language: | English |
Published: |
Elsevier
2015-01-01
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Series: | MethodsX |
Subjects: | |
Online Access: | http://www.sciencedirect.com/science/article/pii/S2215016115000059 |