Optimized Plasmid Construction Strategy for Cas9

Background/Aims: The target genome editing technology not only plays an important role in basic biology studies but also holds a great promise for potential clinical applications. The new generation of engineered nuclease RGEN (RNA Guided EndoNuclease) is much easier to construct and modify, and att...

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Bibliographic Details
Main Authors: Jianyong  Xu, Wenlei Li, MD. Munnaf  Hossen, Yuning Jia, Lingyun Li, Zhong Huang
Format: Article
Language:English
Published: Cell Physiol Biochem Press GmbH & Co KG 2018-07-01
Series:Cellular Physiology and Biochemistry
Subjects:
Online Access:https://www.karger.com/Article/FullText/491669