Bacterial and Eukaryotic Small-Subunit Amplicon Data Do Not Provide a Quantitative Picture of Microbial Communities, but They Are Reliable in the Context of Ecological Interpretations

Bacterial and Eukaryotic Small-Subunit Amplicon Data Do Not Provide a Quantitative Picture of Microbial Communities, but They Are Reliable in the Context of Ecological Interpretations

High-throughput sequencing (HTS) of amplified fragments of rRNA genes provides unprecedented insight into the diversity of prokaryotic and eukaryotic microorganisms. Unfortunately, HTS data are prone to quantitative biases, which may lead to an erroneous picture of microbial community composition an...

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Bibliographic Details
Main Authors: Kasia Piwosz, Tanja Shabarova, Jakob Pernthaler, Thomas Posch, Karel Šimek, Petr Porcal, Michaela M. Salcher
Format: Article
Language:English
Published: American Society for Microbiology 2020-03-01
Series:mSphere
Subjects:
card-fish
amplicon sequencing
bacterial communities
bacterial community structure
bacterial dynamics
eukaryotic communities
eukaryotic community structure
eukaryotic dynamics
microbial abundance
microbial communities
microbial community structure
microbial dynamics
Online Access:https://doi.org/10.1128/mSphere.00052-20

Internet

https://doi.org/10.1128/mSphere.00052-20

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