Bacterial and Eukaryotic Small-Subunit Amplicon Data Do Not Provide a Quantitative Picture of Microbial Communities, but They Are Reliable in the Context of Ecological Interpretations
High-throughput sequencing (HTS) of amplified fragments of rRNA genes provides unprecedented insight into the diversity of prokaryotic and eukaryotic microorganisms. Unfortunately, HTS data are prone to quantitative biases, which may lead to an erroneous picture of microbial community composition an...
Main Authors: | Kasia Piwosz, Tanja Shabarova, Jakob Pernthaler, Thomas Posch, Karel Šimek, Petr Porcal, Michaela M. Salcher |
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Format: | Article |
Language: | English |
Published: |
American Society for Microbiology
2020-03-01
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Series: | mSphere |
Subjects: | |
Online Access: | https://doi.org/10.1128/mSphere.00052-20 |
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