Deciphering the Role of Positions 145 and 165 in Fluorescence Lifetime Shortening in the EGFP Variants

The bright ultimately short lifetime enhanced emitter (BrUSLEE) green fluorescent protein, which differs from the enhanced green fluorescent protein (EGFP) in three mutations, exhibits an extremely short fluorescence lifetime at a relatively high brightness. An important contribution to shortening t...

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Bibliographic Details
Main Authors: Anastasia V. Mamontova, Aleksander M. Shakhov, Konstantin A. Lukyanov, Alexey M. Bogdanov
Format: Article
Language:English
Published: MDPI AG 2020-11-01
Series:Biomolecules
Subjects:
GFP
Online Access:https://www.mdpi.com/2218-273X/10/11/1547