An optimized protocol for microarray validation by quantitative PCR using amplified amino allyl labeled RNA

<p>Abstract</p> <p>Background</p> <p>Validation of microarrays data by quantitative real-time PCR (qPCR) is often limited by the low amount of available RNA. This raised the possibility to perform validation experiments on the amplified amino allyl labeled RNA (AA-aRNA)...

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Bibliographic Details
Main Authors: Wagner Daniel R, Mertes Paul-Michel, Longrois Dan, Jeanty Céline, Devaux Yvan
Format: Article
Language:English
Published: BMC 2010-10-01
Series:BMC Genomics
Online Access:http://www.biomedcentral.com/1471-2164/11/542