High-throughput sequencing of sorted expression libraries reveals inhibitors of bacterial cell division

High-throughput sequencing of sorted expression libraries reveals inhibitors of bacterial cell division

Abstract Background Bacterial filamentation occurs when rod-shaped bacteria grow without dividing. To identify genetically encoded inhibitors of division that promote filamentation, we used cell sorting flow cytometry to enrich filamentous clones from an inducible expression library, and then identi...

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Bibliographic Details
Main Authors: Daniel G. Mediati, Catherine M. Burke, Shirin Ansari, Elizabeth J. Harry, Iain G. Duggin
Format: Article
Language:English
Published: BMC 2018-10-01
Series:BMC Genomics
Subjects:
Cell division
Uropathogenic E. coli
High-throughput
Genetic screen
DNA sequencing
Filamentation
Online Access:http://link.springer.com/article/10.1186/s12864-018-5187-7

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http://link.springer.com/article/10.1186/s12864-018-5187-7

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