Metformin reduces ovarian ischemia reperfusion injury in rats by improving oxidative/nitrosative stress
Objective: To assess the preventive role of metformin on rat ovarian ischemia reperfusion injury. Materials and methods: Forty rats were divided equally into five groups; Group 1: sham, Group 2: surgical control with 3-hr torsion and detorsion, Group 3: 50 mg/kg p.o. metformin 30 min before 3-hr tor...
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doaj-ed31931249ef4753be47f85ed493861d2021-01-24T04:26:34ZengElsevierTaiwanese Journal of Obstetrics & Gynecology1028-45592021-01-016014550Metformin reduces ovarian ischemia reperfusion injury in rats by improving oxidative/nitrosative stressMustafa Demir0Bulent Yilmaz1Senol Kalyoncu2Meltem Tuncer3Zehra Bozdag4Onur Ince5Mehmet Akif Bozdayi6Hasan Ulusal7Seyithan Taysi8Obstetrics and Gynecology Clinic, ANKA Hospital, Gaziantep, Turkey; Corresponding author. Özel Anka Hastanesi, Eyüp Sultan Mahallesi Hafız Tevfik Caddesi No: 162, 27590, Şehitkamil, Gaziantep, Turkey.Department of Obstetrics and Gynecology, Recep Tayyip Erdogan University, Faculty of Medicine, Rize, TurkeyObstetrics and Gynecology Clinic, TOBB ETU Hospital, Ankara, TurkeyDepartment of Physiology, Hacettepe University, Faculty of Medicine, Ankara, TurkeyDepartment of Pathology, Gaziantep University Faculty of Medicine, Gaziantep, TurkeyDepartment of Obstetrics and Gynecology, Kutahya Health Sciences University, Faculty of Medicine, Kutahya, TurkeyDepartment of Biochemistry, Gaziantep University Faculty of Medicine, Gaziantep, TurkeyDepartment of Biochemistry, Gaziantep University Faculty of Medicine, Gaziantep, TurkeyDepartment of Biochemistry, Gaziantep University Faculty of Medicine, Gaziantep, TurkeyObjective: To assess the preventive role of metformin on rat ovarian ischemia reperfusion injury. Materials and methods: Forty rats were divided equally into five groups; Group 1: sham, Group 2: surgical control with 3-hr torsion and detorsion, Group 3: 50 mg/kg p.o. metformin 30 min before 3-hr torsion, Group 4; metformin just after detorsion, Group 5; metformin 30 min before torsion and just after detorsion. Bilateral ovaries and blood sample were obtained seven days after detorsion for biochemical and histopathological evaluation. Results: Ovarian tissue total anti-oxidant status (TAS) levels were significantly increased in group 4 when compared to group 1, 2 and 3 (all p < 0.01). In addition, there was a significant decrease in tissue oxidative stress index (OSI) level in group 4 with respect to group 2 (p < 0.01). Moreover, serum levels of OSI were significantly higher in group 2 with respect to group 1 and 5 (both p < 0.05). Similarly, there was significant increase in serum levels of peroxynitrite in group 2 as compared to serum levels in group 3 and 5 (p < 0.01 and 0.05, respectively). Furthermore, there were significant decrease in histopathological scores metformin and sham groups when compared to rats in the control group (Group 2). Conclusion: Metformin reduces ischemia reperfusion injury in rat torsion detorsion model by improving histopathological and biochemical findings including TAS, OSI and peroxynitrite.http://www.sciencedirect.com/science/article/pii/S1028455920302539Ischemia reperfusion injuryRat ovarian torsionMetforminOxidative stress indexPeroxynitrite |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Mustafa Demir Bulent Yilmaz Senol Kalyoncu Meltem Tuncer Zehra Bozdag Onur Ince Mehmet Akif Bozdayi Hasan Ulusal Seyithan Taysi |
spellingShingle |
Mustafa Demir Bulent Yilmaz Senol Kalyoncu Meltem Tuncer Zehra Bozdag Onur Ince Mehmet Akif Bozdayi Hasan Ulusal Seyithan Taysi Metformin reduces ovarian ischemia reperfusion injury in rats by improving oxidative/nitrosative stress Taiwanese Journal of Obstetrics & Gynecology Ischemia reperfusion injury Rat ovarian torsion Metformin Oxidative stress index Peroxynitrite |
author_facet |
Mustafa Demir Bulent Yilmaz Senol Kalyoncu Meltem Tuncer Zehra Bozdag Onur Ince Mehmet Akif Bozdayi Hasan Ulusal Seyithan Taysi |
author_sort |
Mustafa Demir |
title |
Metformin reduces ovarian ischemia reperfusion injury in rats by improving oxidative/nitrosative stress |
title_short |
Metformin reduces ovarian ischemia reperfusion injury in rats by improving oxidative/nitrosative stress |
title_full |
Metformin reduces ovarian ischemia reperfusion injury in rats by improving oxidative/nitrosative stress |
title_fullStr |
Metformin reduces ovarian ischemia reperfusion injury in rats by improving oxidative/nitrosative stress |
title_full_unstemmed |
Metformin reduces ovarian ischemia reperfusion injury in rats by improving oxidative/nitrosative stress |
title_sort |
metformin reduces ovarian ischemia reperfusion injury in rats by improving oxidative/nitrosative stress |
publisher |
Elsevier |
series |
Taiwanese Journal of Obstetrics & Gynecology |
issn |
1028-4559 |
publishDate |
2021-01-01 |
description |
Objective: To assess the preventive role of metformin on rat ovarian ischemia reperfusion injury. Materials and methods: Forty rats were divided equally into five groups; Group 1: sham, Group 2: surgical control with 3-hr torsion and detorsion, Group 3: 50 mg/kg p.o. metformin 30 min before 3-hr torsion, Group 4; metformin just after detorsion, Group 5; metformin 30 min before torsion and just after detorsion. Bilateral ovaries and blood sample were obtained seven days after detorsion for biochemical and histopathological evaluation. Results: Ovarian tissue total anti-oxidant status (TAS) levels were significantly increased in group 4 when compared to group 1, 2 and 3 (all p < 0.01). In addition, there was a significant decrease in tissue oxidative stress index (OSI) level in group 4 with respect to group 2 (p < 0.01). Moreover, serum levels of OSI were significantly higher in group 2 with respect to group 1 and 5 (both p < 0.05). Similarly, there was significant increase in serum levels of peroxynitrite in group 2 as compared to serum levels in group 3 and 5 (p < 0.01 and 0.05, respectively). Furthermore, there were significant decrease in histopathological scores metformin and sham groups when compared to rats in the control group (Group 2). Conclusion: Metformin reduces ischemia reperfusion injury in rat torsion detorsion model by improving histopathological and biochemical findings including TAS, OSI and peroxynitrite. |
topic |
Ischemia reperfusion injury Rat ovarian torsion Metformin Oxidative stress index Peroxynitrite |
url |
http://www.sciencedirect.com/science/article/pii/S1028455920302539 |
work_keys_str_mv |
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