Development and Experimental Testing of an Optical Micro-Spectroscopic Technique Incorporating True Line-Scan Excitation

Development and Experimental Testing of an Optical Micro-Spectroscopic Technique Incorporating True Line-Scan Excitation

Multiphoton micro-spectroscopy, employing diffraction optics and electron-multiplying CCD (EMCCD) cameras, is a suitable method for determining protein complex stoichiometry, quaternary structure, and spatial distribution in living cells using Förster resonance energy transfer (FRET) imaging. The m...

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Bibliographic Details
Main Authors: Gabriel Biener, Michael R. Stoneman, Gheorghe Acbas, Jessica D. Holz, Marianna Orlova, Liudmila Komarova, Sergei Kuchin, Valerică Raicu
Format: Article
Language:English
Published: MDPI AG 2013-12-01
Series:International Journal of Molecular Sciences
Subjects:
optical micro-spectroscopy
fluorescence
two-photon excitation
multi-photon excitation
energy transfer
Online Access:http://www.mdpi.com/1422-0067/15/1/261

Internet

http://www.mdpi.com/1422-0067/15/1/261

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