Atomic resolution structure of a protein prepared by non-enzymatic His-tag removal. Crystallographic and NMR study of GmSPI-2 inhibitor.

Atomic resolution structure of a protein prepared by non-enzymatic His-tag removal. Crystallographic and NMR study of GmSPI-2 inhibitor.

Purification of suitable quantity of homogenous protein is very often the bottleneck in protein structural studies. Overexpression of a desired gene and attachment of enzymatically cleavable affinity tags to the protein of interest made a breakthrough in this field. Here we describe the structure of...

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Bibliographic Details
Main Authors: Edyta Kopera, Wojciech Bal, Martina Lenarčič Živkovič, Angela Dvornyk, Barbara Kludkiewicz, Krystyna Grzelak, Igor Zhukov, Włodzimierz Zagórski-Ostoja, Mariusz Jaskolski, Szymon Krzywda
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2014-01-01
Series:PLoS ONE
Online Access:https://doi.org/10.1371/journal.pone.0106936

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https://doi.org/10.1371/journal.pone.0106936

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