VLDL hydrolysis by LPL activates PPAR-α through generation of unbound fatty acids

• Main Authors: Maxwell A. Ruby, Benjamin Goldenson, Gabriela Orasanu, Thomas P. Johnston, Jorge Plutzky, Ronald M. Krauss
• Format: Article
• Language: English
• Published: Elsevier 2010-08-01
• Series: Journal of Lipid Research
• Subjects: peroxisome proliferator activated receptor; triglyceride-rich lipoprotein; lipoprotein lipase; apolipoprotein CIII; nonesterified fatty acid; very low-density lipoprotein
• Online Access: http://www.sciencedirect.com/science/article/pii/S0022227520370632

Recent evidence suggests that lipoproteins serve as circulating reservoirs of peroxisomal proliferator activated receptor (PPAR) ligands that are accessible through lipolysis. The present study was conducted to determine the biochemical basis of PPAR-α activation by lipolysis products and their contribution to PPAR-α function in vivo. PPAR-α activation was measured in bovine aortic endothelial cells following treatment with human plasma, VLDL lipolysis products, or oleic acid. While plasma failed to activate PPAR-α, oleic acid performed similarly to VLDL lipolysis products. Therefore, fatty acids are likely to be the PPAR-α ligands generated by VLDL lipolysis. Indeed, unbound fatty acid concentration determined PPAR-α activation regardless of fatty acid source, with PPAR-α activation occurring only at unbound fatty acid concentrations that are unachievable under physiological conditions without lipase action. In mice, a synthetic lipase inhibitor (poloxamer-407) attenuated fasting-induced changes in expression of PPAR-α target genes. Apolipoprotein CIII (apoCIII), an endogenous inhibitor of lipoprotein and hepatic lipase, regulated access to the lipoprotein pool of PPAR-α ligands, because addition of exogenous apoCIII inhibited, and removal of endogenous apoCIII potentiated, lipolytic PPAR-α activation. These data suggest that the PPAR-α response is generated by unbound fatty acids released locally by lipase activity and not by circulating plasma fatty acids.