Neuroprotective Effect of α-Mangostin in the Ameliorating Propionic Acid-Induced Experimental Model of Autism in Wistar Rats
Several studies have documented the role of hyper-activation of extracellular signal-regulated kinases (ERK) in Autism pathogenesis. Alpha-mangostin (AMG) is a phytoconstituents with anti-oxidants, anti-inflammatory, and ERK inhibition properties in many diseases. Our research aims to investigate th...
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doaj-ed9315821ae8409982342ab4fbba87182021-02-26T00:07:25ZengMDPI AGBrain Sciences2076-34252021-02-011128828810.3390/brainsci11030288Neuroprotective Effect of α-Mangostin in the Ameliorating Propionic Acid-Induced Experimental Model of Autism in Wistar RatsAarti Tiwari0Rishabh Khera1Saloni Rahi2Sidharth Mehan3Hafiz Antar Makeen4Yahya H. Khormi5Muneeb U Rehman6Andleeb Khan7Department of Pharmacology, Neuropharmacology Division, ISF College of Pharmacy, Moga, Punjab 142001, IndiaDepartment of Pharmacology, Neuropharmacology Division, ISF College of Pharmacy, Moga, Punjab 142001, IndiaDepartment of Pharmacology, Neuropharmacology Division, ISF College of Pharmacy, Moga, Punjab 142001, IndiaDepartment of Pharmacology, Neuropharmacology Division, ISF College of Pharmacy, Moga, Punjab 142001, IndiaDepartment of Clinical Pharmacy, College of Pharmacy, Jazan University, Jazan 45142, Saudi ArabiaDivision of Neurosurgery, Department of Surgery, Faculty of Medicine, Jazan University, Jazan, 45142, Saudi ArabiaDepartment of Clinical Pharmacy, College of Pharmacy, King Saud University, Riyadh, 11451, Saudi ArabiaDepartment of Pharmacology & Toxicology, College of Pharmacy, Jazan University, Jazan 45142, Saudi ArabiaSeveral studies have documented the role of hyper-activation of extracellular signal-regulated kinases (ERK) in Autism pathogenesis. Alpha-mangostin (AMG) is a phytoconstituents with anti-oxidants, anti-inflammatory, and ERK inhibition properties in many diseases. Our research aims to investigate the neuroprotective effect of AMG in the rat model of intracerebroventricular-propionic acid (ICV-PPA) induced autism with a confirmation of its effect on the ERK signaling. Autism was induced in Wistar rats (total 36 rats; 18 male/18 female) by multiple doses of PPA through ICV injection for 11 days. Actophotometer and beam walking tasks were used to evaluate animals' motor abilities, and the Morris water maze task was utilized to confirm the cognition and memory in animals. Long term administration of AMG 100 mg/kg and AMG 200mg/kg continued from day 12 to day 44 of the experiment. Before that, animals were sacrificed, brains isolated, morphological, gross pathological studies were performed, and neurochemical analysis was performed in the brain homogenates. Cellular and molecular markers, including ERK, myelin basic protein, apoptotic markers including caspase-3, Bax, Bcl-2, neuroinflammatory markers, neurotransmitters, and oxidative stress markers, have been tested throughout the brain. Thus, AMG reduces the overactivation of the ERK signaling and also restored autism-like behavioral and neurochemical alterations.https://www.mdpi.com/2076-3425/11/3/288Autism spectrum disorderERK/mitogen activated protein kinase (MAPK)α-mangostinpropionic acidneuroexcitationgenetic dysfunction |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Aarti Tiwari Rishabh Khera Saloni Rahi Sidharth Mehan Hafiz Antar Makeen Yahya H. Khormi Muneeb U Rehman Andleeb Khan |
spellingShingle |
Aarti Tiwari Rishabh Khera Saloni Rahi Sidharth Mehan Hafiz Antar Makeen Yahya H. Khormi Muneeb U Rehman Andleeb Khan Neuroprotective Effect of α-Mangostin in the Ameliorating Propionic Acid-Induced Experimental Model of Autism in Wistar Rats Brain Sciences Autism spectrum disorder ERK/mitogen activated protein kinase (MAPK) α-mangostin propionic acid neuroexcitation genetic dysfunction |
author_facet |
Aarti Tiwari Rishabh Khera Saloni Rahi Sidharth Mehan Hafiz Antar Makeen Yahya H. Khormi Muneeb U Rehman Andleeb Khan |
author_sort |
Aarti Tiwari |
title |
Neuroprotective Effect of α-Mangostin in the Ameliorating Propionic Acid-Induced Experimental Model of Autism in Wistar Rats |
title_short |
Neuroprotective Effect of α-Mangostin in the Ameliorating Propionic Acid-Induced Experimental Model of Autism in Wistar Rats |
title_full |
Neuroprotective Effect of α-Mangostin in the Ameliorating Propionic Acid-Induced Experimental Model of Autism in Wistar Rats |
title_fullStr |
Neuroprotective Effect of α-Mangostin in the Ameliorating Propionic Acid-Induced Experimental Model of Autism in Wistar Rats |
title_full_unstemmed |
Neuroprotective Effect of α-Mangostin in the Ameliorating Propionic Acid-Induced Experimental Model of Autism in Wistar Rats |
title_sort |
neuroprotective effect of α-mangostin in the ameliorating propionic acid-induced experimental model of autism in wistar rats |
publisher |
MDPI AG |
series |
Brain Sciences |
issn |
2076-3425 |
publishDate |
2021-02-01 |
description |
Several studies have documented the role of hyper-activation of extracellular signal-regulated kinases (ERK) in Autism pathogenesis. Alpha-mangostin (AMG) is a phytoconstituents with anti-oxidants, anti-inflammatory, and ERK inhibition properties in many diseases. Our research aims to investigate the neuroprotective effect of AMG in the rat model of intracerebroventricular-propionic acid (ICV-PPA) induced autism with a confirmation of its effect on the ERK signaling. Autism was induced in Wistar rats (total 36 rats; 18 male/18 female) by multiple doses of PPA through ICV injection for 11 days. Actophotometer and beam walking tasks were used to evaluate animals' motor abilities, and the Morris water maze task was utilized to confirm the cognition and memory in animals. Long term administration of AMG 100 mg/kg and AMG 200mg/kg continued from day 12 to day 44 of the experiment. Before that, animals were sacrificed, brains isolated, morphological, gross pathological studies were performed, and neurochemical analysis was performed in the brain homogenates. Cellular and molecular markers, including ERK, myelin basic protein, apoptotic markers including caspase-3, Bax, Bcl-2, neuroinflammatory markers, neurotransmitters, and oxidative stress markers, have been tested throughout the brain. Thus, AMG reduces the overactivation of the ERK signaling and also restored autism-like behavioral and neurochemical alterations. |
topic |
Autism spectrum disorder ERK/mitogen activated protein kinase (MAPK) α-mangostin propionic acid neuroexcitation genetic dysfunction |
url |
https://www.mdpi.com/2076-3425/11/3/288 |
work_keys_str_mv |
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