Isolation and functional analysis of peridroplet mitochondria from murine brown adipose tissue

Summary: Mitochondria play a central role in lipid metabolism and can bind to lipid droplets. However, the role and functional specialization of the population of peridroplet mitochondria (PDMs) remain unclear, as methods to isolate functional PDMs were not developed until recently. Here, we describ...

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Bibliographic Details
Main Authors: Jennifer Ngo, Ilan Y. Benador, Alexandra J. Brownstein, Laurent Vergnes, Michaela Veliova, Michael Shum, Rebeca Acín-Pérez, Karen Reue, Orian S. Shirihai, Marc Liesa
Format: Article
Language:English
Published: Elsevier 2021-03-01
Series:STAR Protocols
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166720302306