Optimization of an RNA-Seq Differential Gene Expression Analysis Depending on Biological Replicate Number and Library Size

Optimization of an RNA-Seq Differential Gene Expression Analysis Depending on Biological Replicate Number and Library Size

RNA-Seq is a widely used technology that allows an efficient genome-wide quantification of gene expressions for, for example, differential expression (DE) analysis. After a brief review of the main issues, methods and tools related to the DE analysis of RNA-Seq data, this article focuses on the impa...

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Bibliographic Details
Main Authors: Sophie Lamarre, Pierre Frasse, Mohamed Zouine, Delphine Labourdette, Elise Sainderichin, Guojian Hu, Véronique Le Berre-Anton, Mondher Bouzayen, Elie Maza
Format: Article
Language:English
Published: Frontiers Media S.A. 2018-02-01
Series:Frontiers in Plant Science
Subjects:
transcriptomics
RNA-Seq
biological replicates
library size
differential gene expression analysis
power
Online Access:http://journal.frontiersin.org/article/10.3389/fpls.2018.00108/full

Internet

http://journal.frontiersin.org/article/10.3389/fpls.2018.00108/full

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