Application of long single-stranded DNA donors in genome editing: generation and validation of mouse mutants

Application of long single-stranded DNA donors in genome editing: generation and validation of mouse mutants

Abstract Background Recent advances in clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 (Cas9) genome editing have led to the use of long single-stranded DNA (lssDNA) molecules for generating conditional mutations. However, there is still limited availab...

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Bibliographic Details
Main Authors: Gemma F. Codner, Joffrey Mianné, Adam Caulder, Jorik Loeffler, Rachel Fell, Ruairidh King, Alasdair J. Allan, Matthew Mackenzie, Fran J. Pike, Christopher V. McCabe, Skevoulla Christou, Sam Joynson, Marie Hutchison, Michelle E. Stewart, Saumya Kumar, Michelle M. Simon, Loranne Agius, Quentin M. Anstee, Kirill E. Volynski, Dimitri M. Kullmann, Sara Wells, Lydia Teboul
Format: Article
Language:English
Published: BMC 2018-06-01
Series:BMC Biology
Subjects:
Allele validation
Conditional
CRISPR/Cas9
Homologous recombination
Mouse
Mutant
Online Access:http://link.springer.com/article/10.1186/s12915-018-0530-7

Internet

http://link.springer.com/article/10.1186/s12915-018-0530-7

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