Visualising leukocyte trafficking in the living brain with 2-photon intravital microscopy

Intravital imaging of the superficial brain tissue in mice represents a powerful tool for the dissection of the cellular and molecular cues underlying inflammatory and infectious central nervous system diseases. We present here a step-by-step protocol that will enable a non-specialist to set up a tw...

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Bibliographic Details
Main Authors: Saparna ePai, Karyn J Danne, Jim eQin, Lois L Cavanagh, Adrian eSmith, Michael J Hickey, Wolfgang eWeninger
Format: Article
Language:English
Published: Frontiers Media S.A. 2013-01-01
Series:Frontiers in Cellular Neuroscience
Subjects:
Online Access:http://journal.frontiersin.org/Journal/10.3389/fncel.2012.00067/full