An att site-based recombination reporter system for genome engineering and synthetic DNA assembly
Abstract Background Direct manipulation of the genome is a widespread technique for genetic studies and synthetic biology applications. The tyrosine and serine site-specific recombination systems of bacteriophages HK022 and ΦC31 are widely used for stable directional exchange and relocation of DNA s...
Main Authors: | , , , |
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Format: | Article |
Language: | English |
Published: |
BMC
2017-07-01
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Series: | BMC Biotechnology |
Subjects: | |
Online Access: | http://link.springer.com/article/10.1186/s12896-017-0382-1 |