An att site-based recombination reporter system for genome engineering and synthetic DNA assembly

Abstract Background Direct manipulation of the genome is a widespread technique for genetic studies and synthetic biology applications. The tyrosine and serine site-specific recombination systems of bacteriophages HK022 and ΦC31 are widely used for stable directional exchange and relocation of DNA s...

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Bibliographic Details
Main Authors: Michael J. Bland, Magaly Ducos-Galand, Marie-Eve Val, Didier Mazel
Format: Article
Language:English
Published: BMC 2017-07-01
Series:BMC Biotechnology
Subjects:
Online Access:http://link.springer.com/article/10.1186/s12896-017-0382-1