EEF1D overexpression promotes osteosarcoma cell proliferation by facilitating Akt-mTOR and Akt-bad signaling
Abstract Background Dysregulation of eukaryotic translation elongation factor 1 delta (EEF1D) in cancers has been reported; however, the role and mechanisms of EEF1D in osteosarcoma remain poorly understood. The aim of this study is to investigate the expression and role of EEF1D in osteosarcoma and...
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doaj-ee9485b70efe4f4d843c3532543a6fd32020-11-25T00:24:50ZengBMCJournal of Experimental & Clinical Cancer Research1756-99662018-03-0137111010.1186/s13046-018-0715-5EEF1D overexpression promotes osteosarcoma cell proliferation by facilitating Akt-mTOR and Akt-bad signalingDong-dong Cheng0Shi-jie Li1Bin Zhu2Shu-min Zhou3Qing-cheng Yang4Department of Orthopedics, Shanghai Jiao Tong University Affiliated Sixth People’s HospitalDepartment of Orthopedics, Shanghai Jiao Tong University Affiliated Sixth People’s HospitalDepartment of Orthopedics, Shanghai Jiao Tong University Affiliated Sixth People’s HospitalInstitution of microsurgery for limbs, Shanghai Jiao Tong University Affiliated Sixth People’s HospitalDepartment of Orthopedics, Shanghai Jiao Tong University Affiliated Sixth People’s HospitalAbstract Background Dysregulation of eukaryotic translation elongation factor 1 delta (EEF1D) in cancers has been reported; however, the role and mechanisms of EEF1D in osteosarcoma remain poorly understood. The aim of this study is to investigate the expression and role of EEF1D in osteosarcoma and to elucidate its underlying mechanisms. Methods The expression of EEF1D in osteosarcomas and cell lines was evaluated by qRT-PCR, Western blotting and immunohistochemistry. EEF1D knockdown using small interfering RNA (siRNA) was employed to analyze the role of EEF1D in osteosarcoma cell proliferation and cell cycle progression. The host signaling pathways affected by EEF1D knockdown were detected using PathScan® intracellular signaling array kit. Results The expression of EEF1D was found to be up-regulated in human osteosarcoma tissues and cell lines. Its expression was positively correlated with Enneking stage and the tumor recurrence. EEF1D knockdown inhibited osteosarcoma cell proliferation, colony-forming ability, and cell cycle G2/M transition in vitro. In addition, EEF1D knockdown decreased the levels of phospho-Akt, phospho-mTOR, and phospho-Bad proteins. Conclusions EEF1D is upregulated in osteosarcoma and plays a tumor promoting role by facilitating Akt-mTOR and Akt-Bad signaling pathways. Accordingly, EEF1D is a potential target for cancer therapy.http://link.springer.com/article/10.1186/s13046-018-0715-5EEF1DProliferationAkt-mTOR signaling pathwayAkt-bad signaling pathwayOsteosarcoma |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Dong-dong Cheng Shi-jie Li Bin Zhu Shu-min Zhou Qing-cheng Yang |
spellingShingle |
Dong-dong Cheng Shi-jie Li Bin Zhu Shu-min Zhou Qing-cheng Yang EEF1D overexpression promotes osteosarcoma cell proliferation by facilitating Akt-mTOR and Akt-bad signaling Journal of Experimental & Clinical Cancer Research EEF1D Proliferation Akt-mTOR signaling pathway Akt-bad signaling pathway Osteosarcoma |
author_facet |
Dong-dong Cheng Shi-jie Li Bin Zhu Shu-min Zhou Qing-cheng Yang |
author_sort |
Dong-dong Cheng |
title |
EEF1D overexpression promotes osteosarcoma cell proliferation by facilitating Akt-mTOR and Akt-bad signaling |
title_short |
EEF1D overexpression promotes osteosarcoma cell proliferation by facilitating Akt-mTOR and Akt-bad signaling |
title_full |
EEF1D overexpression promotes osteosarcoma cell proliferation by facilitating Akt-mTOR and Akt-bad signaling |
title_fullStr |
EEF1D overexpression promotes osteosarcoma cell proliferation by facilitating Akt-mTOR and Akt-bad signaling |
title_full_unstemmed |
EEF1D overexpression promotes osteosarcoma cell proliferation by facilitating Akt-mTOR and Akt-bad signaling |
title_sort |
eef1d overexpression promotes osteosarcoma cell proliferation by facilitating akt-mtor and akt-bad signaling |
publisher |
BMC |
series |
Journal of Experimental & Clinical Cancer Research |
issn |
1756-9966 |
publishDate |
2018-03-01 |
description |
Abstract Background Dysregulation of eukaryotic translation elongation factor 1 delta (EEF1D) in cancers has been reported; however, the role and mechanisms of EEF1D in osteosarcoma remain poorly understood. The aim of this study is to investigate the expression and role of EEF1D in osteosarcoma and to elucidate its underlying mechanisms. Methods The expression of EEF1D in osteosarcomas and cell lines was evaluated by qRT-PCR, Western blotting and immunohistochemistry. EEF1D knockdown using small interfering RNA (siRNA) was employed to analyze the role of EEF1D in osteosarcoma cell proliferation and cell cycle progression. The host signaling pathways affected by EEF1D knockdown were detected using PathScan® intracellular signaling array kit. Results The expression of EEF1D was found to be up-regulated in human osteosarcoma tissues and cell lines. Its expression was positively correlated with Enneking stage and the tumor recurrence. EEF1D knockdown inhibited osteosarcoma cell proliferation, colony-forming ability, and cell cycle G2/M transition in vitro. In addition, EEF1D knockdown decreased the levels of phospho-Akt, phospho-mTOR, and phospho-Bad proteins. Conclusions EEF1D is upregulated in osteosarcoma and plays a tumor promoting role by facilitating Akt-mTOR and Akt-Bad signaling pathways. Accordingly, EEF1D is a potential target for cancer therapy. |
topic |
EEF1D Proliferation Akt-mTOR signaling pathway Akt-bad signaling pathway Osteosarcoma |
url |
http://link.springer.com/article/10.1186/s13046-018-0715-5 |
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