Cumulative mitochondrial activity correlates with ototoxin susceptibility in zebrafish mechanosensory hair cells
Mitochondria play a prominent role in mechanosensory hair cell damage and death. Although hair cells are thought to be energetically demanding cells, how mitochondria respond to these demands and how this might relate to cell death is largely unexplored. Using genetically encoded indicators, we foun...
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doaj-eebd6114d51347f0a4d4899207f158bc2021-05-05T16:23:44ZengeLife Sciences Publications LtdeLife2050-084X2018-12-01710.7554/eLife.38062Cumulative mitochondrial activity correlates with ototoxin susceptibility in zebrafish mechanosensory hair cellsSarah B Pickett0https://orcid.org/0000-0003-1657-1660Eric D Thomas1Joy Y Sebe2Tor Linbo3Robert Esterberg4Dale W Hailey5David W Raible6https://orcid.org/0000-0002-5342-5841Department of Biological Structure, University of Washington, Seattle, United States; Graduate Program in Neuroscience, University of Washington, Seattle, United StatesDepartment of Biological Structure, University of Washington, Seattle, United States; Graduate Program in Neuroscience, University of Washington, Seattle, United StatesDepartment of Biological Structure, University of Washington, Seattle, United StatesDepartment of Biological Structure, University of Washington, Seattle, United StatesDepartment of Biological Structure, University of Washington, Seattle, United States; Virginia Merrill Bloedel Hearing Research Center, University of Washington, Seattle, United StatesDepartment of Biological Structure, University of Washington, Seattle, United States; Virginia Merrill Bloedel Hearing Research Center, University of Washington, Seattle, United StatesDepartment of Biological Structure, University of Washington, Seattle, United States; Graduate Program in Neuroscience, University of Washington, Seattle, United States; Virginia Merrill Bloedel Hearing Research Center, University of Washington, Seattle, United StatesMitochondria play a prominent role in mechanosensory hair cell damage and death. Although hair cells are thought to be energetically demanding cells, how mitochondria respond to these demands and how this might relate to cell death is largely unexplored. Using genetically encoded indicators, we found that mitochondrial calcium flux and oxidation are regulated by mechanotransduction and demonstrate that hair cell activity has both acute and long-term consequences on mitochondrial function. We tested whether variation in mitochondrial activity reflected differences in the vulnerability of hair cells to the toxic drug neomycin. We observed that susceptibility did not correspond to the acute level of mitochondrial activity but rather to the cumulative history of that activity.https://elifesciences.org/articles/38062lateral linehair cellmitochondria |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Sarah B Pickett Eric D Thomas Joy Y Sebe Tor Linbo Robert Esterberg Dale W Hailey David W Raible |
spellingShingle |
Sarah B Pickett Eric D Thomas Joy Y Sebe Tor Linbo Robert Esterberg Dale W Hailey David W Raible Cumulative mitochondrial activity correlates with ototoxin susceptibility in zebrafish mechanosensory hair cells eLife lateral line hair cell mitochondria |
author_facet |
Sarah B Pickett Eric D Thomas Joy Y Sebe Tor Linbo Robert Esterberg Dale W Hailey David W Raible |
author_sort |
Sarah B Pickett |
title |
Cumulative mitochondrial activity correlates with ototoxin susceptibility in zebrafish mechanosensory hair cells |
title_short |
Cumulative mitochondrial activity correlates with ototoxin susceptibility in zebrafish mechanosensory hair cells |
title_full |
Cumulative mitochondrial activity correlates with ototoxin susceptibility in zebrafish mechanosensory hair cells |
title_fullStr |
Cumulative mitochondrial activity correlates with ototoxin susceptibility in zebrafish mechanosensory hair cells |
title_full_unstemmed |
Cumulative mitochondrial activity correlates with ototoxin susceptibility in zebrafish mechanosensory hair cells |
title_sort |
cumulative mitochondrial activity correlates with ototoxin susceptibility in zebrafish mechanosensory hair cells |
publisher |
eLife Sciences Publications Ltd |
series |
eLife |
issn |
2050-084X |
publishDate |
2018-12-01 |
description |
Mitochondria play a prominent role in mechanosensory hair cell damage and death. Although hair cells are thought to be energetically demanding cells, how mitochondria respond to these demands and how this might relate to cell death is largely unexplored. Using genetically encoded indicators, we found that mitochondrial calcium flux and oxidation are regulated by mechanotransduction and demonstrate that hair cell activity has both acute and long-term consequences on mitochondrial function. We tested whether variation in mitochondrial activity reflected differences in the vulnerability of hair cells to the toxic drug neomycin. We observed that susceptibility did not correspond to the acute level of mitochondrial activity but rather to the cumulative history of that activity. |
topic |
lateral line hair cell mitochondria |
url |
https://elifesciences.org/articles/38062 |
work_keys_str_mv |
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1721459370951180288 |