Single-Step Gene Knockout of the SUC2 Gene in Saccharomyces cerevisiae: A Laboratory Exercise for Undergraduate Students
This article describes a relatively straightforward procedure to knock out the gene that encodes the invertase enzyme in baker’s yeast. The SUC2 gene, which encodes for the invertase enzyme, is knocked out by a single-step PCR knock out method. The knockout is subsequently confirmed at the genetic...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
American Society for Microbiology
2019-01-01
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| Series: | Journal of Microbiology & Biology Education |
| Subjects: | |
| Online Access: | http://jmbesubmissions.asm.org/index.php/jmbe/article/view/1615 |