Redirecting substrate regioselectivity using engineered ΔN123-GBD-CD2 branching sucrases for the production of pentasaccharide repeating units of S. flexneri 3a, 4a and 4b haptens

Redirecting substrate regioselectivity using engineered ΔN123-GBD-CD2 branching sucrases for the production of pentasaccharide repeating units of S. flexneri 3a, 4a and 4b haptens

Abstract The (chemo-)enzymatic synthesis of oligosaccharides has been hampered by the lack of appropriate enzymatic tools with requisite regio- and stereo-specificities. Engineering of carbohydrate-active enzymes, in particular targeting the enzyme active site, has notably led to catalysts with alte...

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Bibliographic Details
Main Authors: Mounir Benkoulouche, Akli Ben Imeddourene, Louis-Antoine Barel, Guillaume Le Heiget, Sandra Pizzut, Hanna Kulyk, Floriant Bellvert, Sophie Bozonnet, Laurence A. Mulard, Magali Remaud-Siméon, Claire Moulis, Isabelle André
Format: Article
Language:English
Published: Nature Publishing Group 2021-01-01
Series:Scientific Reports
Online Access:https://doi.org/10.1038/s41598-021-81719-1

Internet

https://doi.org/10.1038/s41598-021-81719-1

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