Probiotic Properties of Exopolysaccharide-Producing Lactobacillus Strains Isolated from Tempoyak
Tempoyak is a functional Malaysian food (an acid-fermented condiment) which is produced from the pulp of the durian (Durio zibethinus) fruit. The current study aimed to isolate and identify potential exopolysaccharide (EPS)-producing Lactobacillus strains from tempoyak for potential use as probiotic...
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doaj-ef3486c9f86b4292adc9fe96500065972020-11-24T23:05:57ZengMDPI AGMolecules1420-30492018-02-0123239810.3390/molecules23020398molecules23020398Probiotic Properties of Exopolysaccharide-Producing Lactobacillus Strains Isolated from TempoyakEilaf Suliman Khalil0Mohd Yazid Abd Manap1Shuhaimi Mustafa2Amaal M. Alhelli3Parisa Shokryazdan4Department of Food Technology, Faculty of Food Science and Technology, Universiti Putra Malaysia, Serdang 43400 UPM, Selangor, MalaysiaDepartment of Food Technology, Faculty of Food Science and Technology, Universiti Putra Malaysia, Serdang 43400 UPM, Selangor, MalaysiaHalal Products Research Institute, Universiti Putra Malaysia, Serdang 43400 UPM, Selangor, MalaysiaDepartment of Food Science, Faculty of Food Science and Technology, Universiti Putra Malaysia, Serdang 43400 UPM, Selangor, MalaysiaAgriculture Biotechnology Research Institute of Iran (ABRII), East and North-East Branch, P.O. Box 91735/844, Mashhad, IranTempoyak is a functional Malaysian food (an acid-fermented condiment) which is produced from the pulp of the durian (Durio zibethinus) fruit. The current study aimed to isolate and identify potential exopolysaccharide (EPS)-producing Lactobacillus strains from tempoyak for potential use as probiotics. Seven isolates (DUR2, DUR4, DUR5, DUR8, DUR12, DUR18, and DUR20) out of 44 were able to produce EPS, and exhibited resistance to acid and bile salt compared to the reference strains Lactobacillus rhmnosus (ATCC53103) and L. plantarum (ATCC8014). The seven isolated strains belonged to five different species—L. plantarum, L. fermentum, L. crispatus, L. reuteri, and L. pentosus—which were identified using API 50 CHL and 16S rRNA gene sequences (Polymerase chain reaction, PCR – based). The seven strains displayed different ability to produce EPS (100–850 mg/L). Isolates exhibited a high survivability to acid (pH 3.0), bile salts (0.3%), and gastrointestinal tract model (<70%). Results showed that the auto-aggregation and cell surface hydrophobicity ranged from 39.98% to 60.09% and 50.80% to 80.53%, respectively, whereas, the highest co-aggregation value (66.44%) was observed by L. fermentum (DUR8) with Pseudomonas aeruginosa. The isolates showed good inhibitory activity against tested pathogens, high antioxidant activity (32.29% to 73.36%), and good ability to reduce cholesterol (22.55% to 75.15%). Thus, the seven tested strains have value as probiotics.http://www.mdpi.com/1420-3049/23/2/398antibacterial activityLactobacillusexopolysaccharidesprobioticstempoyak |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Eilaf Suliman Khalil Mohd Yazid Abd Manap Shuhaimi Mustafa Amaal M. Alhelli Parisa Shokryazdan |
spellingShingle |
Eilaf Suliman Khalil Mohd Yazid Abd Manap Shuhaimi Mustafa Amaal M. Alhelli Parisa Shokryazdan Probiotic Properties of Exopolysaccharide-Producing Lactobacillus Strains Isolated from Tempoyak Molecules antibacterial activity Lactobacillus exopolysaccharides probiotics tempoyak |
author_facet |
Eilaf Suliman Khalil Mohd Yazid Abd Manap Shuhaimi Mustafa Amaal M. Alhelli Parisa Shokryazdan |
author_sort |
Eilaf Suliman Khalil |
title |
Probiotic Properties of Exopolysaccharide-Producing Lactobacillus Strains Isolated from Tempoyak |
title_short |
Probiotic Properties of Exopolysaccharide-Producing Lactobacillus Strains Isolated from Tempoyak |
title_full |
Probiotic Properties of Exopolysaccharide-Producing Lactobacillus Strains Isolated from Tempoyak |
title_fullStr |
Probiotic Properties of Exopolysaccharide-Producing Lactobacillus Strains Isolated from Tempoyak |
title_full_unstemmed |
Probiotic Properties of Exopolysaccharide-Producing Lactobacillus Strains Isolated from Tempoyak |
title_sort |
probiotic properties of exopolysaccharide-producing lactobacillus strains isolated from tempoyak |
publisher |
MDPI AG |
series |
Molecules |
issn |
1420-3049 |
publishDate |
2018-02-01 |
description |
Tempoyak is a functional Malaysian food (an acid-fermented condiment) which is produced from the pulp of the durian (Durio zibethinus) fruit. The current study aimed to isolate and identify potential exopolysaccharide (EPS)-producing Lactobacillus strains from tempoyak for potential use as probiotics. Seven isolates (DUR2, DUR4, DUR5, DUR8, DUR12, DUR18, and DUR20) out of 44 were able to produce EPS, and exhibited resistance to acid and bile salt compared to the reference strains Lactobacillus rhmnosus (ATCC53103) and L. plantarum (ATCC8014). The seven isolated strains belonged to five different species—L. plantarum, L. fermentum, L. crispatus, L. reuteri, and L. pentosus—which were identified using API 50 CHL and 16S rRNA gene sequences (Polymerase chain reaction, PCR – based). The seven strains displayed different ability to produce EPS (100–850 mg/L). Isolates exhibited a high survivability to acid (pH 3.0), bile salts (0.3%), and gastrointestinal tract model (<70%). Results showed that the auto-aggregation and cell surface hydrophobicity ranged from 39.98% to 60.09% and 50.80% to 80.53%, respectively, whereas, the highest co-aggregation value (66.44%) was observed by L. fermentum (DUR8) with Pseudomonas aeruginosa. The isolates showed good inhibitory activity against tested pathogens, high antioxidant activity (32.29% to 73.36%), and good ability to reduce cholesterol (22.55% to 75.15%). Thus, the seven tested strains have value as probiotics. |
topic |
antibacterial activity Lactobacillus exopolysaccharides probiotics tempoyak |
url |
http://www.mdpi.com/1420-3049/23/2/398 |
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