A Cas9 Variant for Efficient Generation of Indel-Free Knockin or Gene-Corrected Human Pluripotent Stem Cells

A Cas9 Variant for Efficient Generation of Indel-Free Knockin or Gene-Corrected Human Pluripotent Stem Cells

While Cas9 nucleases permit rapid and efficient generation of gene-edited cell lines, the CRISPR-Cas9 system can introduce undesirable “on-target” mutations within the second allele of successfully modified cells via non-homologous end joining (NHEJ). To address this, we fused the Streptococcus pyog...

Full description

Bibliographic Details
Main Authors: Sara E. Howden, Bradley McColl, Astrid Glaser, Jim Vadolas, Steven Petrou, Melissa H. Little, Andrew G. Elefanty, Edouard G. Stanley
Format: Article
Language:English
Published: Elsevier 2016-09-01
Series:Stem Cell Reports
Online Access:http://www.sciencedirect.com/science/article/pii/S2213671116301060

Internet

http://www.sciencedirect.com/science/article/pii/S2213671116301060

Similar Items