Anti-TRBC1 Antibody-Based Flow Cytometric Detection of T-Cell Clonality: Standardization of Sample Preparation and Diagnostic Implementation

Anti-TRBC1 Antibody-Based Flow Cytometric Detection of T-Cell Clonality: Standardization of Sample Preparation and Diagnostic Implementation

A single antibody (anti-TRBC1; JOVI-1 antibody clone) against one of the two mutually exclusive T-cell receptor β-chain constant domains was identified as a potentially useful flow-cytometry (FCM) marker to assess Tαβ-cell clonality. We optimized the TRBC1-FCM approach for detecting clonal Tαβ-cells...

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Bibliographic Details
Main Authors: Noemí Muñoz-García, Margarida Lima, Neus Villamor, F. Javier Morán-Plata, Susana Barrena, Sheila Mateos, Carolina Caldas, Ana Balanzategui, Miguel Alcoceba, Alejandro Domínguez, Fabio Gómez, Anton W. Langerak, Jacques J. M. van Dongen, Alberto Orfao, Julia Almeida
Format: Article
Language:English
Published: MDPI AG 2021-08-01
Series:Cancers
Subjects:
TRBC1
JOVI-1
T-CLPD
Tαβ-cells
TRBJ1 and TRBJ2
TCRVβ
Online Access:https://www.mdpi.com/2072-6694/13/17/4379

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https://www.mdpi.com/2072-6694/13/17/4379

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