Synergistic Apoptotic Effect Of Indole-3-Carbinol And Doxorubicin On Pre-B Acute Lymphoblastic Leukemia Cells
Background and Aim: Indole-3-carbinol (I3C), found in Brassica species vegetables, exhibits antitumor effects. It has been shown that I3C induces apoptosis in various cell types through inactivation of the nuclear factor-kappa B (NF- k B) pathway. Anthracyclines such as doxorubicin, is widely used i...
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Tehran University of Medical Sciences
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doaj-f01f712a25b840a0a2c79494685c4aaa2021-10-02T17:32:09ZfasTehran University of Medical Sciencesپیاورد سلامت1735-81322008-26652015-01-0185368378Synergistic Apoptotic Effect Of Indole-3-Carbinol And Doxorubicin On Pre-B Acute Lymphoblastic Leukemia CellsBehnaz Tavasoli0Majid Safa1Ahmad Kazemi2 Master of Sciences in Hematology & Blood Banking, School of Allied Medicine, Iran University of Medical Sciences, Tehran, Iran Assistant Professor, Hematology & Blood Banking Department, School of Allied Medicine, Cellular and Molecular Research Center, Iran University of Medical Sciences, Tehran, Iran Professor, Hematology & Blood Banking Department, School of Allied Medicine, Iran University of Medical Sciences, Tehran, Iran Background and Aim: Indole-3-carbinol (I3C), found in Brassica species vegetables, exhibits antitumor effects. It has been shown that I3C induces apoptosis in various cell types through inactivation of the nuclear factor-kappa B (NF- k B) pathway. Anthracyclines such as doxorubicin, is widely used in the treatment of hematological malignancies, induce apoptosis in tumor cells via DNA damage and activation of p53. However, NF- k B pathway that activated by anthracyclines as a part of DNA damage response can induce chemo resistance. In this study the apoptotic effect of doxorubicin in combination with NF- k B inhibitor I3C was assessed in acute lymphoblastic leukemia cells. Materials and Methods: Human pre-B acute lymphoblastic leukemia cell line, NALM-6 cells, were preincubated with various concentrations of I3C for1 hour and then treated with 125nM doxorubicin at 37 ° C for 24 hours. Cellular DNA content assay and Annexin V-FITC staining were performed by flowcytometry for evaluation of apoptosis. Results: DNA histogram analysis of NALM-6 cells indicates that combination of I3C with doxorubicin synergistically escalated the percentages of sub-G1 population cells (apoptotic cells) as compared to doxorubicin-only treated group. Annexin V-FITC staining showed that cotreatment of NALM-6 cells with I3C and doxorubicin increased the proportion of Annexin-V positive cells (early apoptotic cells) in comparison with the doxorubicin treated cells. Conclusion: The results of cell culture treatments and cell death analysis by flowcytometry suggest that I3C synergistically potentiates doxorubicin-induced apoptosis in human leukemia NALM-6 cells.http://payavard.tums.ac.ir/article-1-5529-en.htmli3capoptosisdoxorubicinacute lymphoblastic leukemia |
collection |
DOAJ |
language |
fas |
format |
Article |
sources |
DOAJ |
author |
Behnaz Tavasoli Majid Safa Ahmad Kazemi |
spellingShingle |
Behnaz Tavasoli Majid Safa Ahmad Kazemi Synergistic Apoptotic Effect Of Indole-3-Carbinol And Doxorubicin On Pre-B Acute Lymphoblastic Leukemia Cells پیاورد سلامت i3c apoptosis doxorubicin acute lymphoblastic leukemia |
author_facet |
Behnaz Tavasoli Majid Safa Ahmad Kazemi |
author_sort |
Behnaz Tavasoli |
title |
Synergistic Apoptotic Effect Of Indole-3-Carbinol And Doxorubicin On Pre-B Acute Lymphoblastic Leukemia Cells |
title_short |
Synergistic Apoptotic Effect Of Indole-3-Carbinol And Doxorubicin On Pre-B Acute Lymphoblastic Leukemia Cells |
title_full |
Synergistic Apoptotic Effect Of Indole-3-Carbinol And Doxorubicin On Pre-B Acute Lymphoblastic Leukemia Cells |
title_fullStr |
Synergistic Apoptotic Effect Of Indole-3-Carbinol And Doxorubicin On Pre-B Acute Lymphoblastic Leukemia Cells |
title_full_unstemmed |
Synergistic Apoptotic Effect Of Indole-3-Carbinol And Doxorubicin On Pre-B Acute Lymphoblastic Leukemia Cells |
title_sort |
synergistic apoptotic effect of indole-3-carbinol and doxorubicin on pre-b acute lymphoblastic leukemia cells |
publisher |
Tehran University of Medical Sciences |
series |
پیاورد سلامت |
issn |
1735-8132 2008-2665 |
publishDate |
2015-01-01 |
description |
Background and Aim: Indole-3-carbinol (I3C), found in Brassica species vegetables, exhibits antitumor effects. It has been shown that I3C induces apoptosis in various cell types through inactivation of the nuclear factor-kappa B (NF- k B) pathway. Anthracyclines such as doxorubicin, is widely used in the treatment of hematological malignancies, induce apoptosis in tumor cells via DNA damage and activation of p53. However, NF- k B pathway that activated by anthracyclines as a part of DNA damage response can induce chemo resistance. In this study the apoptotic effect of doxorubicin in combination with NF- k B inhibitor I3C was assessed in acute lymphoblastic leukemia cells. Materials and Methods: Human pre-B acute lymphoblastic leukemia cell line, NALM-6 cells, were preincubated with various concentrations of I3C for1 hour and then treated with 125nM doxorubicin at 37 ° C for 24 hours. Cellular DNA content assay and Annexin V-FITC staining were performed by flowcytometry for evaluation of apoptosis. Results: DNA histogram analysis of NALM-6 cells indicates that combination of I3C with doxorubicin synergistically escalated the percentages of sub-G1 population cells (apoptotic cells) as compared to doxorubicin-only treated group. Annexin V-FITC staining showed that cotreatment of NALM-6 cells with I3C and doxorubicin increased the proportion of Annexin-V positive cells (early apoptotic cells) in comparison with the doxorubicin treated cells. Conclusion: The results of cell culture treatments and cell death analysis by flowcytometry suggest that I3C synergistically potentiates doxorubicin-induced apoptosis in human leukemia NALM-6 cells. |
topic |
i3c apoptosis doxorubicin acute lymphoblastic leukemia |
url |
http://payavard.tums.ac.ir/article-1-5529-en.html |
work_keys_str_mv |
AT behnaztavasoli synergisticapoptoticeffectofindole3carbinolanddoxorubicinonprebacutelymphoblasticleukemiacells AT majidsafa synergisticapoptoticeffectofindole3carbinolanddoxorubicinonprebacutelymphoblasticleukemiacells AT ahmadkazemi synergisticapoptoticeffectofindole3carbinolanddoxorubicinonprebacutelymphoblasticleukemiacells |
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1716851379305185280 |