Fermented Sea Tangle (<i>Laminaria japonica</i> Aresch) Suppresses RANKL-Induced Osteoclastogenesis by Scavenging ROS in RAW 264.7 Cells
Sea tangle (<i>Laminaria japonica</i> Aresch), a brown alga, has been used for many years as a functional food ingredient in the Asia-Pacific region. In the present study, we investigated the effects of fermented sea tangle extract (FST) on receptor activator of nuclear factor-κ...
Main Authors: | , , , , , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
MDPI AG
2019-07-01
|
Series: | Foods |
Subjects: | |
Online Access: | https://www.mdpi.com/2304-8158/8/8/290 |
id |
doaj-f022abffafa3427da40756ee5e439d7e |
---|---|
record_format |
Article |
spelling |
doaj-f022abffafa3427da40756ee5e439d7e2020-11-25T01:34:01ZengMDPI AGFoods2304-81582019-07-018829010.3390/foods8080290foods8080290Fermented Sea Tangle (<i>Laminaria japonica</i> Aresch) Suppresses RANKL-Induced Osteoclastogenesis by Scavenging ROS in RAW 264.7 CellsJin-Woo Jeong0Seon Yeong Ji1Hyesook Lee2Su Hyun Hong3Gi-Young Kim4Cheol Park5Bae-Jin Lee6Eui Kyun Park7Jin Won Hyun8You-Jin Jeon9Yung Hyun Choi10Nakdonggang National Institute of Biological Resources, Sangju 37242, KoreaDepartment of Biochemistry, Dong-eui University College of Korean Medicine, Busan 47227, KoreaDepartment of Biochemistry, Dong-eui University College of Korean Medicine, Busan 47227, KoreaDepartment of Biochemistry, Dong-eui University College of Korean Medicine, Busan 47227, KoreaDepartment of Marine Life Sciences, Jeju National University, Jeju 63243, KoreaDepartment of Molecular Biology, College of Natural Sciences, Dong-eui University, Busan 47340, KoreaMarine Bioprocess Co. Ltd., Busan 46048, KoreaDepartment of Oral Pathology and Regenerative Medicine, School of Dentistry, Kyungpook National University, Daegu 41940, KoreaDepartment of Biochemistry, School of Medicine, Jeju National University, Jeju 63243, KoreaDepartment of Marine Life Sciences, Jeju National University, Jeju 63243, KoreaDepartment of Biochemistry, Dong-eui University College of Korean Medicine, Busan 47227, KoreaSea tangle (<i>Laminaria japonica</i> Aresch), a brown alga, has been used for many years as a functional food ingredient in the Asia-Pacific region. In the present study, we investigated the effects of fermented sea tangle extract (FST) on receptor activator of nuclear factor-κB (NF-κB) ligand (RANKL)-stimulated osteoclast differentiation, using RAW 264.7 mouse macrophage cells. FST was found to inhibit the RANKL-stimulated activation of tartrate-resistance acid phosphatase (TRAP) and F-actin ring structure formation. FST also down-regulated the expression of osteoclast marker genes like TRAP, matrix metalloproteinase-9, cathepsin K and osteoclast-associated receptor by blocking RANKL-induced activation of NF-κB and expression of nuclear factor of activated T cells c1 (NFATc1), a master transcription factor. In addition, FST significantly abolished RANKL-induced generation of reactive oxygen species (ROS) by activation of nuclear factor-erythroid 2-related factor 2 (Nrf2) and its transcriptional targets. Hence, it seems likely that FST may have anti-osteoclastogenic potential as a result of its ability to inactivate the NF-κB-mediated NFATc1 signaling pathway and by reducing ROS production through activation of the Nrf2 pathway. Although further studies are needed to inquire its efficacy in vivo, FST appears to have potential use as an adjunctive or as a prophylactic treatment for osteoclastic bone disease.https://www.mdpi.com/2304-8158/8/8/290fermented sea tangleosteoclast differentiationreceptor of activator of nuclear factor kappa-B ligand (RANKL)nuclear factor-κB (NF-κB)reactive oxygen species (ROS) |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Jin-Woo Jeong Seon Yeong Ji Hyesook Lee Su Hyun Hong Gi-Young Kim Cheol Park Bae-Jin Lee Eui Kyun Park Jin Won Hyun You-Jin Jeon Yung Hyun Choi |
spellingShingle |
Jin-Woo Jeong Seon Yeong Ji Hyesook Lee Su Hyun Hong Gi-Young Kim Cheol Park Bae-Jin Lee Eui Kyun Park Jin Won Hyun You-Jin Jeon Yung Hyun Choi Fermented Sea Tangle (<i>Laminaria japonica</i> Aresch) Suppresses RANKL-Induced Osteoclastogenesis by Scavenging ROS in RAW 264.7 Cells Foods fermented sea tangle osteoclast differentiation receptor of activator of nuclear factor kappa-B ligand (RANKL) nuclear factor-κB (NF-κB) reactive oxygen species (ROS) |
author_facet |
Jin-Woo Jeong Seon Yeong Ji Hyesook Lee Su Hyun Hong Gi-Young Kim Cheol Park Bae-Jin Lee Eui Kyun Park Jin Won Hyun You-Jin Jeon Yung Hyun Choi |
author_sort |
Jin-Woo Jeong |
title |
Fermented Sea Tangle (<i>Laminaria japonica</i> Aresch) Suppresses RANKL-Induced Osteoclastogenesis by Scavenging ROS in RAW 264.7 Cells |
title_short |
Fermented Sea Tangle (<i>Laminaria japonica</i> Aresch) Suppresses RANKL-Induced Osteoclastogenesis by Scavenging ROS in RAW 264.7 Cells |
title_full |
Fermented Sea Tangle (<i>Laminaria japonica</i> Aresch) Suppresses RANKL-Induced Osteoclastogenesis by Scavenging ROS in RAW 264.7 Cells |
title_fullStr |
Fermented Sea Tangle (<i>Laminaria japonica</i> Aresch) Suppresses RANKL-Induced Osteoclastogenesis by Scavenging ROS in RAW 264.7 Cells |
title_full_unstemmed |
Fermented Sea Tangle (<i>Laminaria japonica</i> Aresch) Suppresses RANKL-Induced Osteoclastogenesis by Scavenging ROS in RAW 264.7 Cells |
title_sort |
fermented sea tangle (<i>laminaria japonica</i> aresch) suppresses rankl-induced osteoclastogenesis by scavenging ros in raw 264.7 cells |
publisher |
MDPI AG |
series |
Foods |
issn |
2304-8158 |
publishDate |
2019-07-01 |
description |
Sea tangle (<i>Laminaria japonica</i> Aresch), a brown alga, has been used for many years as a functional food ingredient in the Asia-Pacific region. In the present study, we investigated the effects of fermented sea tangle extract (FST) on receptor activator of nuclear factor-κB (NF-κB) ligand (RANKL)-stimulated osteoclast differentiation, using RAW 264.7 mouse macrophage cells. FST was found to inhibit the RANKL-stimulated activation of tartrate-resistance acid phosphatase (TRAP) and F-actin ring structure formation. FST also down-regulated the expression of osteoclast marker genes like TRAP, matrix metalloproteinase-9, cathepsin K and osteoclast-associated receptor by blocking RANKL-induced activation of NF-κB and expression of nuclear factor of activated T cells c1 (NFATc1), a master transcription factor. In addition, FST significantly abolished RANKL-induced generation of reactive oxygen species (ROS) by activation of nuclear factor-erythroid 2-related factor 2 (Nrf2) and its transcriptional targets. Hence, it seems likely that FST may have anti-osteoclastogenic potential as a result of its ability to inactivate the NF-κB-mediated NFATc1 signaling pathway and by reducing ROS production through activation of the Nrf2 pathway. Although further studies are needed to inquire its efficacy in vivo, FST appears to have potential use as an adjunctive or as a prophylactic treatment for osteoclastic bone disease. |
topic |
fermented sea tangle osteoclast differentiation receptor of activator of nuclear factor kappa-B ligand (RANKL) nuclear factor-κB (NF-κB) reactive oxygen species (ROS) |
url |
https://www.mdpi.com/2304-8158/8/8/290 |
work_keys_str_mv |
AT jinwoojeong fermentedseatangleilaminariajaponicaiareschsuppressesranklinducedosteoclastogenesisbyscavengingrosinraw2647cells AT seonyeongji fermentedseatangleilaminariajaponicaiareschsuppressesranklinducedosteoclastogenesisbyscavengingrosinraw2647cells AT hyesooklee fermentedseatangleilaminariajaponicaiareschsuppressesranklinducedosteoclastogenesisbyscavengingrosinraw2647cells AT suhyunhong fermentedseatangleilaminariajaponicaiareschsuppressesranklinducedosteoclastogenesisbyscavengingrosinraw2647cells AT giyoungkim fermentedseatangleilaminariajaponicaiareschsuppressesranklinducedosteoclastogenesisbyscavengingrosinraw2647cells AT cheolpark fermentedseatangleilaminariajaponicaiareschsuppressesranklinducedosteoclastogenesisbyscavengingrosinraw2647cells AT baejinlee fermentedseatangleilaminariajaponicaiareschsuppressesranklinducedosteoclastogenesisbyscavengingrosinraw2647cells AT euikyunpark fermentedseatangleilaminariajaponicaiareschsuppressesranklinducedosteoclastogenesisbyscavengingrosinraw2647cells AT jinwonhyun fermentedseatangleilaminariajaponicaiareschsuppressesranklinducedosteoclastogenesisbyscavengingrosinraw2647cells AT youjinjeon fermentedseatangleilaminariajaponicaiareschsuppressesranklinducedosteoclastogenesisbyscavengingrosinraw2647cells AT yunghyunchoi fermentedseatangleilaminariajaponicaiareschsuppressesranklinducedosteoclastogenesisbyscavengingrosinraw2647cells |
_version_ |
1725074200730796032 |