A simpler and more cost-effective peptide biosynthetic method using the truncated GST as carrier for epitope mapping.

There is a need to develop better methods for epitope mapping and/or identification of antibody-recognizing motifs. Here, we describe improved biosynthetic peptide (BSP) method using a newly developed plasmid pXXGST-3 as vector, which has a viral E7 gene in the cloning sites of pXXGST-1. It is cruci...

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Bibliographic Details
Main Authors: Wan-Xiang Xu, Jian Wang, Hai-Ping Tang, Ling-Han Chen, Wen-Bo Lian, Jian-Min Zhan, Satish K Gupta, Chao-Neng Ji, Shao-Hua Gu, Yi Xie
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2017-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC5638316?pdf=render