Concentration of Rat Bone Marrow Nucleated Cells Using Hypo-osmotic Hemolysis in Distilled Water

Background: Bone marrow aspirates contain primarily red blood cells. To achieve efficient cell transplantation for regeneration, the red blood cells need to be removed from the aspirates. Cell isolation is typically performed using density gradient centrifugation. However, this method entails issues...

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Bibliographic Details
Main Authors: Fumio Ohnishi, MD, Hiroko Ochiai, MD, PhD, Kazuo Kishi, MD, PhD
Format: Article
Language:English
Published: Wolters Kluwer 2014-07-01
Series:Plastic and Reconstructive Surgery, Global Open
Online Access:http://journals.lww.com/prsgo/Fulltext/2014/07000/Article.6.aspx