pH dependence of non-specific adsorption and detection solution in electrochemical metalloimmunoassay using antibody–silver nanoparticle conjugates

The pH dependence of non-specific adsorption of antibody–silver nanoparticle conjugates (AgNPs–Ab) to an antibody-immobilized electrode was investigated. The adsorption was promoted at lower pH values (pH 3.0) and inhibited at higher pH values. These results are attributed to the zeta potential of t...

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Bibliographic Details
Main Authors: Nobuyasu Hori, Miyuki Chikae, Hiroya Kirimura, Yuzuru Takamura
Format: Article
Language:English
Published: Elsevier 2015-09-01
Series:Sensing and Bio-Sensing Research
Online Access:http://www.sciencedirect.com/science/article/pii/S2214180415300027