FMRP regulates the subcellular distribution of cortical dendritic spine density in a non-cell-autonomous manner
Fragile X syndrome (FXS) is the most common form of intellectual disability that arises from the dysfunction of a single gene—Fmr1. The main neuroanatomical correlate of FXS is elevated dendritic spine density on cortical pyramidal neurons, which has been modeled in Fmr1-/Y mice. However, the cell-a...
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doaj-f093f93ed884470caf2820e987f841b02021-03-22T08:43:04ZengElsevierNeurobiology of Disease1095-953X2021-03-01150105253FMRP regulates the subcellular distribution of cortical dendritic spine density in a non-cell-autonomous mannerKatherine M. Bland0Adam Aharon1Eden L. Widener2M. Irene Song3Zachary O. Casey4Yi Zuo5George S. Vidal6Department of Biology, James Madison University, Harrisonburg, VA 22801, United StatesDepartment of Molecular, Cell and Developmental Biology, University of California Santa Cruz, Santa Cruz, CA 95064, United StatesDepartment of Biology, James Madison University, Harrisonburg, VA 22801, United StatesDepartment of Biology, James Madison University, Harrisonburg, VA 22801, United StatesDepartment of Biology, James Madison University, Harrisonburg, VA 22801, United StatesDepartment of Molecular, Cell and Developmental Biology, University of California Santa Cruz, Santa Cruz, CA 95064, United States; Corresponding authors.Department of Biology, James Madison University, Harrisonburg, VA 22801, United States; Corresponding authors.Fragile X syndrome (FXS) is the most common form of intellectual disability that arises from the dysfunction of a single gene—Fmr1. The main neuroanatomical correlate of FXS is elevated dendritic spine density on cortical pyramidal neurons, which has been modeled in Fmr1-/Y mice. However, the cell-autonomous contribution of Fmr1 on cortical dendritic spine density has not been assessed. Even less is known about the role of Fmr1 in heterozygous female mosaic mice, which are a putative model for human Fmr1 full mutation carriers (i.e., are heterozygous for the full Fmr1-silencing mutation). In this neuroanatomical study, spine density in cortical pyramidal neurons of Fmr1+/− and Fmr1-/Y mice was studied at multiple subcellular compartments, layers, and brain regions. Spine density in Fmr1+/− mice is higher than WT but lower than Fmr1-/Y. Not all subcellular compartments in layer V Fmr1+/− and Fmr1-/Y cortical pyramidal neurons are equally affected: the apical dendrite, a key subcellular compartment, is principally affected over basal dendrites. Within apical dendrites, spine density is differentially affected across branch orders. Finally, identification of FMRP-positive and FMRP-negative neurons within Fmr1+/− permitted the study of the cell-autonomous effect of Fmr1 on spine density. Surprisingly, layer V cortical pyramidal spine density between FMRP-positive and FMRP-negative neurons does not differ, suggesting that the regulation of the primary neuroanatomical defect of FXS—elevated spine density—is non-cell-autonomous.http://www.sciencedirect.com/science/article/pii/S0969996121000024Fragile X SyndromeFmr1Dendritic spineDendriteLayer VLayer 5 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Katherine M. Bland Adam Aharon Eden L. Widener M. Irene Song Zachary O. Casey Yi Zuo George S. Vidal |
spellingShingle |
Katherine M. Bland Adam Aharon Eden L. Widener M. Irene Song Zachary O. Casey Yi Zuo George S. Vidal FMRP regulates the subcellular distribution of cortical dendritic spine density in a non-cell-autonomous manner Neurobiology of Disease Fragile X Syndrome Fmr1 Dendritic spine Dendrite Layer V Layer 5 |
author_facet |
Katherine M. Bland Adam Aharon Eden L. Widener M. Irene Song Zachary O. Casey Yi Zuo George S. Vidal |
author_sort |
Katherine M. Bland |
title |
FMRP regulates the subcellular distribution of cortical dendritic spine density in a non-cell-autonomous manner |
title_short |
FMRP regulates the subcellular distribution of cortical dendritic spine density in a non-cell-autonomous manner |
title_full |
FMRP regulates the subcellular distribution of cortical dendritic spine density in a non-cell-autonomous manner |
title_fullStr |
FMRP regulates the subcellular distribution of cortical dendritic spine density in a non-cell-autonomous manner |
title_full_unstemmed |
FMRP regulates the subcellular distribution of cortical dendritic spine density in a non-cell-autonomous manner |
title_sort |
fmrp regulates the subcellular distribution of cortical dendritic spine density in a non-cell-autonomous manner |
publisher |
Elsevier |
series |
Neurobiology of Disease |
issn |
1095-953X |
publishDate |
2021-03-01 |
description |
Fragile X syndrome (FXS) is the most common form of intellectual disability that arises from the dysfunction of a single gene—Fmr1. The main neuroanatomical correlate of FXS is elevated dendritic spine density on cortical pyramidal neurons, which has been modeled in Fmr1-/Y mice. However, the cell-autonomous contribution of Fmr1 on cortical dendritic spine density has not been assessed. Even less is known about the role of Fmr1 in heterozygous female mosaic mice, which are a putative model for human Fmr1 full mutation carriers (i.e., are heterozygous for the full Fmr1-silencing mutation). In this neuroanatomical study, spine density in cortical pyramidal neurons of Fmr1+/− and Fmr1-/Y mice was studied at multiple subcellular compartments, layers, and brain regions. Spine density in Fmr1+/− mice is higher than WT but lower than Fmr1-/Y. Not all subcellular compartments in layer V Fmr1+/− and Fmr1-/Y cortical pyramidal neurons are equally affected: the apical dendrite, a key subcellular compartment, is principally affected over basal dendrites. Within apical dendrites, spine density is differentially affected across branch orders. Finally, identification of FMRP-positive and FMRP-negative neurons within Fmr1+/− permitted the study of the cell-autonomous effect of Fmr1 on spine density. Surprisingly, layer V cortical pyramidal spine density between FMRP-positive and FMRP-negative neurons does not differ, suggesting that the regulation of the primary neuroanatomical defect of FXS—elevated spine density—is non-cell-autonomous. |
topic |
Fragile X Syndrome Fmr1 Dendritic spine Dendrite Layer V Layer 5 |
url |
http://www.sciencedirect.com/science/article/pii/S0969996121000024 |
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