Cloning, optimization of periplasmic expression and purification of recombinant granulocyte macrophage-stimulating factor in Escherichia coli BL21 (DE3)

Cloning, optimization of periplasmic expression and purification of recombinant granulocyte macrophage-stimulating factor in Escherichia coli BL21 (DE3)

Background: Molgramostim, a nonglycosylated version of recombinant granulocyte-macrophage colony-stimulating factor (GM-CSF), can be produced in a high level by Escherichia coli. However, overexpression of GM-CSF in bacterial cells usually leads to formation of inclusion bodies and insoluble protein...

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Bibliographic Details
Main Authors: Elham Taherian, Elmira Mohammadi, Ali Jahanian-Najafabadi, Fatemeh Moazen, Vajihe Akbari
Format: Article
Language:English
Published: Wolters Kluwer Medknow Publications 2019-01-01
Series:Advanced Biomedical Research
Subjects:
escherichia coli
granulocyte-macrophage colony-stimulating factor
inclusion bodies
periplasmic expression
Online Access:http://www.advbiores.net/article.asp?issn=2277-9175;year=2019;volume=8;issue=1;spage=71;epage=71;aulast=Taherian

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http://www.advbiores.net/article.asp?issn=2277-9175;year=2019;volume=8;issue=1;spage=71;epage=71;aulast=Taherian

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