Thermostabilization of indigenous multiplex polymerase chain reaction reagents for detection of enterotoxigenic Staphylococcus aureus

Background/Purpose: Among DNA-based techniques, polymerase chain reaction (PCR) is the most widely accepted molecular tool for the detection of pathogens. However, the technique involves several reagents and multiple pipetting steps that often lead to error-prone results. Additionally, the reagents...

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Bibliographic Details
Main Authors: Sowmya Nagaraj, Shylaja Ramlal, Joseph Kingston, Harsh Vardhan Batra
Format: Article
Language:English
Published: Elsevier 2018-04-01
Series:Journal of Microbiology, Immunology and Infection
Online Access:http://www.sciencedirect.com/science/article/pii/S1684118216300330