Next-generation sequencing for hypothesis-free genomic detection of invasive tropical infections in poly-microbially contaminated, formalin-fixed, paraffin-embedded tissue samples – a proof-of-principle assessment

Next-generation sequencing for hypothesis-free genomic detection of invasive tropical infections in poly-microbially contaminated, formalin-fixed, paraffin-embedded tissue samples – a proof-of-principle assessment

Abstract Background The potential of next-generation sequencing (NGS) for hypothesis-free pathogen diagnosis from (poly-)microbially contaminated, formalin-fixed, paraffin embedded tissue samples from patients with invasive fungal infections and amebiasis was investigated. Samples from patients with...

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Bibliographic Details
Main Authors: Hagen Frickmann, Carsten Künne, Ralf Matthias Hagen, Andreas Podbielski, Jana Normann, Sven Poppert, Mario Looso, Bernd Kreikemeyer
Format: Article
Language:English
Published: BMC 2019-04-01
Series:BMC Microbiology
Subjects:
NGS
Next-generation sequencing
Hypothesis-free diagnosis of infection
Invasive fungal infections
Invasive amebiasis
FFPE, formalin-fixed, paraffin-embedded samples
Online Access:http://link.springer.com/article/10.1186/s12866-019-1448-0

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http://link.springer.com/article/10.1186/s12866-019-1448-0

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