Genomic GC-Content Affects the Accuracy of 16S rRNA Gene Sequencing Based Microbial Profiling due to PCR Bias

Genomic GC-Content Affects the Accuracy of 16S rRNA Gene Sequencing Based Microbial Profiling due to PCR Bias

Profiling of microbial community composition is frequently performed by partial 16S rRNA gene sequencing on benchtop platforms following PCR amplification of specific hypervariable regions within this gene. Accuracy and reproducibility of this strategy are two key parameters to consider, which may b...

Full description

Bibliographic Details
Main Authors: Martin F. Laursen, Marlene D. Dalgaard, Martin I. Bahl
Format: Article
Language:English
Published: Frontiers Media S.A. 2017-10-01
Series:Frontiers in Microbiology
Subjects:
ion torrent PGM
16S rRNA gene sequencing
reproducibility
accuracy
mock community
genomic GC content
Online Access:http://journal.frontiersin.org/article/10.3389/fmicb.2017.01934/full

Internet

http://journal.frontiersin.org/article/10.3389/fmicb.2017.01934/full

Similar Items