Clinical evaluation of a low cost, in-house developed real-time RT-PCR human immunodeficiency virus type 1 (HIV-1) quantitation assay for HIV-1 infected patients.

Clinical evaluation of a low cost, in-house developed real-time RT-PCR human immunodeficiency virus type 1 (HIV-1) quantitation assay for HIV-1 infected patients.

OBJECTIVES: HIV-1 viral quantitation is essential for treatment monitoring. An in-house assay would decrease financial barriers to access. MATERIALS AND METHODS: A real-time competitive RT-PCR in house assay (Sing-IH) was developed in Singapore. Using HXB2 as reference, the assay's primers and...

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Main Authors: Palvinder Kaur, Wei Xin Khong, Sue Yuen Wee, Eng Lee Tan, Juergen Pipper, Evelyn Koay, Kah Ying Ng, Joe Kwan Yap, Kuan Kiat Chew, Mei Ting Tan, Yee Sin Leo, Masafumi Inoue, Oon Tek Ng
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2014-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC3945479?pdf=render

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http://europepmc.org/articles/PMC3945479?pdf=render

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