Purification of Cyclospora cayetanensis oocysts obtained from human stool specimens for whole genome sequencing

Purification of Cyclospora cayetanensis oocysts obtained from human stool specimens for whole genome sequencing

Abstract Background Cyclospora cayetanensis is a food-borne intestinal human parasite that causes outbreaks of diarrhea. There is a need for efficient laboratory methods for strain-level characterization to assist in outbreak investigations. By using next generation sequencing, genomic sequences can...

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Bibliographic Details
Main Authors: Yvonne Qvarnstrom, Yuping Wei-Pridgeon, Erik Van Roey, Subin Park, Ganesh Srinivasamoorthy, Fernanda S. Nascimento, Delynn M. Moss, Eldin Talundzic, Michael J. Arrowood
Format: Article
Language:English
Published: BMC 2018-10-01
Series:Gut Pathogens
Subjects:
Whole genome sequencing
Next generation sequencing
Cyclospora cayetanensis
Density gradient separation
Flow cytometry sorting
Oocysts
Online Access:http://link.springer.com/article/10.1186/s13099-018-0272-7

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http://link.springer.com/article/10.1186/s13099-018-0272-7

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