Design, Development and Optimization of a Functional Mammalian Cell-Free Protein Synthesis Platform

Design, Development and Optimization of a Functional Mammalian Cell-Free Protein Synthesis Platform

In this paper, we describe the stepwise development of a cell-free protein synthesis (CFPS) platform derived from cultured Chinese hamster ovary (CHO) cells. We provide a retrospective summary of the design challenges we faced, and the optimized methods developed for the cultivation of cells and the...

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Bibliographic Details
Main Authors: Chiara Heide, Gizem Buldum, Ignacio Moya-Ramirez, Oscar Ces, Cleo Kontoravdi, Karen M. Polizzi
Format: Article
Language:English
Published: Frontiers Media S.A. 2021-02-01
Series:Frontiers in Bioengineering and Biotechnology
Subjects:
cell-free protein synthesis
Chinese hamster ovary cells
cell extract
synthetic biology
in vitro transcription-translation
coupled batch reactions
Online Access:https://www.frontiersin.org/articles/10.3389/fbioe.2020.604091/full

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https://www.frontiersin.org/articles/10.3389/fbioe.2020.604091/full

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