Impact of Bead-Beating Intensity on the Genus- and Species-Level Characterization of the Gut Microbiome Using Amplicon and Complete 16S rRNA Gene Sequencing

Impact of Bead-Beating Intensity on the Genus- and Species-Level Characterization of the Gut Microbiome Using Amplicon and Complete 16S rRNA Gene Sequencing

Bead-beating within a DNA extraction protocol is critical for complete microbial cell lysis and accurate assessment of the abundance and composition of the microbiome. While the impact of bead-beating on the recovery of OTUs at the phylum and class level have been studied, its influence on species-l...

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Bibliographic Details
Main Authors: Bo Zhang, Matthew Brock, Carlos Arana, Chaitanya Dende, Nicolai Stanislas van Oers, Lora V. Hooper, Prithvi Raj
Format: Article
Language:English
Published: Frontiers Media S.A. 2021-10-01
Series:Frontiers in Cellular and Infection Microbiology
Subjects:
microbiome
16S
sequencing
DNA extraction
bead-beating
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Online Access:https://www.frontiersin.org/articles/10.3389/fcimb.2021.678522/full

Internet

https://www.frontiersin.org/articles/10.3389/fcimb.2021.678522/full

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