Quantitative profiling of N 6-methyladenosine at single-base resolution in stem-differentiating xylem of Populus trichocarpa using Nanopore direct RNA sequencing

Quantitative profiling of N 6-methyladenosine at single-base resolution in stem-differentiating xylem of Populus trichocarpa using Nanopore direct RNA sequencing

Abstract There are no comprehensive methods to identify N 6-methyladenosine (m6A) at single-base resolution for every single transcript, which is necessary for the estimation of m6A abundance. We develop a new pipeline called Nanom6A for the identification and quantification of m6A modification at s...

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Bibliographic Details
Main Authors: Yubang Gao, Xuqing Liu, Bizhi Wu, Huihui Wang, Feihu Xi, Markus V. Kohnen, Anireddy S. N. Reddy, Lianfeng Gu
Format: Article
Language:English
Published: BMC 2021-01-01
Series:Genome Biology
Subjects:
N 6-Methyladenosine
Nanopore direct RNA sequencing
Alternative polyadenylation
Stem-differentiating xylem
Populus trichocarpa
Online Access:https://doi.org/10.1186/s13059-020-02241-7

Internet

https://doi.org/10.1186/s13059-020-02241-7

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