Effect of Feeding Cold-Pressed Sunflower Cake on Ruminal Fermentation, Lipid Metabolism and Bacterial Community in Dairy Cows
Cold-pressed sunflower cake (CPSC), by-product of oil-manufacturing, has high crude fat and linoleic acid concentrations, being a promising supplement to modulate rumen fatty acid (FA) profile. This trial studied CPSC effects on ruminal fermentation, biohydrogenation and the bacterial community in d...
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doaj-f17a7b07e764414088f681a8192a7aaa2020-11-25T01:27:36ZengMDPI AGAnimals2076-26152019-10-0191075510.3390/ani9100755ani9100755Effect of Feeding Cold-Pressed Sunflower Cake on Ruminal Fermentation, Lipid Metabolism and Bacterial Community in Dairy CowsIzaro Zubiria0Aser Garcia-Rodriguez1Raquel Atxaerandio2Roberto Ruiz3Hanen Benhissi4Nerea Mandaluniz5Jose Luis Lavín6Leticia Abecia7Idoia Goiri8NEIKER-Granja Modelo de Arkaute, Apdo. 46., 01080 Vitoria-Gasteiz, SpainNEIKER-Granja Modelo de Arkaute, Apdo. 46., 01080 Vitoria-Gasteiz, SpainNEIKER-Granja Modelo de Arkaute, Apdo. 46., 01080 Vitoria-Gasteiz, SpainNEIKER-Granja Modelo de Arkaute, Apdo. 46., 01080 Vitoria-Gasteiz, SpainNEIKER-Granja Modelo de Arkaute, Apdo. 46., 01080 Vitoria-Gasteiz, SpainNEIKER-Granja Modelo de Arkaute, Apdo. 46., 01080 Vitoria-Gasteiz, SpainCICbioGUNE. Parque Científico Tecnológico de Bizkaia, Ed. 801A. Derio, 48160 Bizkaia, SpainCICbioGUNE. Parque Científico Tecnológico de Bizkaia, Ed. 801A. Derio, 48160 Bizkaia, SpainNEIKER-Granja Modelo de Arkaute, Apdo. 46., 01080 Vitoria-Gasteiz, SpainCold-pressed sunflower cake (CPSC), by-product of oil-manufacturing, has high crude fat and linoleic acid concentrations, being a promising supplement to modulate rumen fatty acid (FA) profile. This trial studied CPSC effects on ruminal fermentation, biohydrogenation and the bacterial community in dairy cows. Ten cows were used in a crossover design with two experimental diets and fed during two 63-day periods. The cows were group fed forage ad libitum and the concentrate individually. The concentrates, control and CPSC, were isoenergetic, isoproteic and isofat. The ruminal samples collected at the end of each experimental period were analyzed for short-chain fatty acid, FA and DNA sequencing. CPSC decreased butyrate molar proportion (4%, <i>p</i> = 0.005). CPSC decreased C16:0 (28%, <i>p</i> < 0.001) and increased C18:0 (14%, <i>p</i> < 0.001) and total monounsaturated FA, especially C18:1 trans-11 (13%, <i>p</i> = 0.023). The total purine derivative excretion tended to be greater (5%, <i>p</i> = 0.05) with CPSC, resulting in a 6% greater daily microbial N flow. CPSC did not affect the diversity indices but increased the relative abundances of <i>Treponema</i> and <i>Coprococcus</i>, and decreased <i>Enterococcus</i>, <i>Ruminococcus</i> and <i>Succinivibrio</i>. In conclusion, the changes in ruminal fermentation and the FA profile were not associated with changes in microbial diversity or abundance of dominant populations, however, they might be associated with less abundant genera.https://www.mdpi.com/2076-2615/9/10/755biohydrogenationsunflower cakemicrobiota |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Izaro Zubiria Aser Garcia-Rodriguez Raquel Atxaerandio Roberto Ruiz Hanen Benhissi Nerea Mandaluniz Jose Luis Lavín Leticia Abecia Idoia Goiri |
spellingShingle |
Izaro Zubiria Aser Garcia-Rodriguez Raquel Atxaerandio Roberto Ruiz Hanen Benhissi Nerea Mandaluniz Jose Luis Lavín Leticia Abecia Idoia Goiri Effect of Feeding Cold-Pressed Sunflower Cake on Ruminal Fermentation, Lipid Metabolism and Bacterial Community in Dairy Cows Animals biohydrogenation sunflower cake microbiota |
author_facet |
Izaro Zubiria Aser Garcia-Rodriguez Raquel Atxaerandio Roberto Ruiz Hanen Benhissi Nerea Mandaluniz Jose Luis Lavín Leticia Abecia Idoia Goiri |
author_sort |
Izaro Zubiria |
title |
Effect of Feeding Cold-Pressed Sunflower Cake on Ruminal Fermentation, Lipid Metabolism and Bacterial Community in Dairy Cows |
title_short |
Effect of Feeding Cold-Pressed Sunflower Cake on Ruminal Fermentation, Lipid Metabolism and Bacterial Community in Dairy Cows |
title_full |
Effect of Feeding Cold-Pressed Sunflower Cake on Ruminal Fermentation, Lipid Metabolism and Bacterial Community in Dairy Cows |
title_fullStr |
Effect of Feeding Cold-Pressed Sunflower Cake on Ruminal Fermentation, Lipid Metabolism and Bacterial Community in Dairy Cows |
title_full_unstemmed |
Effect of Feeding Cold-Pressed Sunflower Cake on Ruminal Fermentation, Lipid Metabolism and Bacterial Community in Dairy Cows |
title_sort |
effect of feeding cold-pressed sunflower cake on ruminal fermentation, lipid metabolism and bacterial community in dairy cows |
publisher |
MDPI AG |
series |
Animals |
issn |
2076-2615 |
publishDate |
2019-10-01 |
description |
Cold-pressed sunflower cake (CPSC), by-product of oil-manufacturing, has high crude fat and linoleic acid concentrations, being a promising supplement to modulate rumen fatty acid (FA) profile. This trial studied CPSC effects on ruminal fermentation, biohydrogenation and the bacterial community in dairy cows. Ten cows were used in a crossover design with two experimental diets and fed during two 63-day periods. The cows were group fed forage ad libitum and the concentrate individually. The concentrates, control and CPSC, were isoenergetic, isoproteic and isofat. The ruminal samples collected at the end of each experimental period were analyzed for short-chain fatty acid, FA and DNA sequencing. CPSC decreased butyrate molar proportion (4%, <i>p</i> = 0.005). CPSC decreased C16:0 (28%, <i>p</i> < 0.001) and increased C18:0 (14%, <i>p</i> < 0.001) and total monounsaturated FA, especially C18:1 trans-11 (13%, <i>p</i> = 0.023). The total purine derivative excretion tended to be greater (5%, <i>p</i> = 0.05) with CPSC, resulting in a 6% greater daily microbial N flow. CPSC did not affect the diversity indices but increased the relative abundances of <i>Treponema</i> and <i>Coprococcus</i>, and decreased <i>Enterococcus</i>, <i>Ruminococcus</i> and <i>Succinivibrio</i>. In conclusion, the changes in ruminal fermentation and the FA profile were not associated with changes in microbial diversity or abundance of dominant populations, however, they might be associated with less abundant genera. |
topic |
biohydrogenation sunflower cake microbiota |
url |
https://www.mdpi.com/2076-2615/9/10/755 |
work_keys_str_mv |
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