Serum-free and xenobiotic-free preservation of cultured human limbal epithelial cells.

To develop a one-week storage method, without serum and xenobiotics, that would maintain cell viability, morphology, and phenotype of cultured human limbal epithelial sheets.Human limbal explants were cultured on intact human amniotic membranes for two weeks. The sheets were stored in a hermetically...

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Bibliographic Details
Main Authors: Oeygunn Utheim, Rakibul Islam, Torstein Lyberg, Borghild Roald, Jon Roger Eidet, Maria Fideliz de la Paz, Darlene A Dartt, Sten Raeder, Tor Paaske Utheim
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2015-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC4348416?pdf=render